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Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity

The present study was carried out to investigate the antioxidant and neuroprotective effects of Hyptis suaveolens methanol extract (HSME) using various in vitro systems. The total phenol and flavonoids contents of the HSME were quantified by colorimetric methods. The HSME extract exhibited potent an...

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Autores principales: Ghaffari, Hadi, Ghassam, Behrouz Jalali, Chandra Nayaka, S., Ramachandra Kini, K., Prakash, H. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950622/
https://www.ncbi.nlm.nih.gov/pubmed/24420496
http://dx.doi.org/10.1007/s10571-013-0016-7
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author Ghaffari, Hadi
Ghassam, Behrouz Jalali
Chandra Nayaka, S.
Ramachandra Kini, K.
Prakash, H. S.
author_facet Ghaffari, Hadi
Ghassam, Behrouz Jalali
Chandra Nayaka, S.
Ramachandra Kini, K.
Prakash, H. S.
author_sort Ghaffari, Hadi
collection PubMed
description The present study was carried out to investigate the antioxidant and neuroprotective effects of Hyptis suaveolens methanol extract (HSME) using various in vitro systems. The total phenol and flavonoids contents of the HSME were quantified by colorimetric methods. The HSME extract exhibited potent antioxidant activity as determined by 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing antioxidant power assays. The neuroprotective activity of HSME was determined on mouse N2A neuroblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lactate dehydrogenase, intracellular ROS assays, and upregulation of brain neuronal markers at genetic level. The N2A cells were pretreated with different concentrations (0.5, 1, 1.5, and 2 mg/ml) of the extract and then exposed to H(2)O(2) to induce oxidative stress and neurotoxicity. The survival of the cells treated with different concentrations of HSME and H(2)O(2) increased as compared to cells exposed only to H(2)O(2) (47.3 %) (p < 0.05). The HSME also dose-dependently reduced LDH leakage and intracellular ROS production (p < 0.05). Pretreatment with HSME promotes the upregulation of tyrosine hydroxylase (2.41-fold, p < 0.05), and brain-derived neurotrophic factor genes (2.15-fold, p < 0.05) against H(2)O(2)-induced cytotoxicity in N2A cells. Moreover, the HSME showed antioxidant activity and decreased neurotoxicity. These observations suggest that HSME have marked antioxidant and neuroprotective activities.
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spelling pubmed-39506222014-03-20 Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity Ghaffari, Hadi Ghassam, Behrouz Jalali Chandra Nayaka, S. Ramachandra Kini, K. Prakash, H. S. Cell Mol Neurobiol Original Research The present study was carried out to investigate the antioxidant and neuroprotective effects of Hyptis suaveolens methanol extract (HSME) using various in vitro systems. The total phenol and flavonoids contents of the HSME were quantified by colorimetric methods. The HSME extract exhibited potent antioxidant activity as determined by 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing antioxidant power assays. The neuroprotective activity of HSME was determined on mouse N2A neuroblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lactate dehydrogenase, intracellular ROS assays, and upregulation of brain neuronal markers at genetic level. The N2A cells were pretreated with different concentrations (0.5, 1, 1.5, and 2 mg/ml) of the extract and then exposed to H(2)O(2) to induce oxidative stress and neurotoxicity. The survival of the cells treated with different concentrations of HSME and H(2)O(2) increased as compared to cells exposed only to H(2)O(2) (47.3 %) (p < 0.05). The HSME also dose-dependently reduced LDH leakage and intracellular ROS production (p < 0.05). Pretreatment with HSME promotes the upregulation of tyrosine hydroxylase (2.41-fold, p < 0.05), and brain-derived neurotrophic factor genes (2.15-fold, p < 0.05) against H(2)O(2)-induced cytotoxicity in N2A cells. Moreover, the HSME showed antioxidant activity and decreased neurotoxicity. These observations suggest that HSME have marked antioxidant and neuroprotective activities. Springer US 2014-01-14 2014 /pmc/articles/PMC3950622/ /pubmed/24420496 http://dx.doi.org/10.1007/s10571-013-0016-7 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Research
Ghaffari, Hadi
Ghassam, Behrouz Jalali
Chandra Nayaka, S.
Ramachandra Kini, K.
Prakash, H. S.
Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity
title Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity
title_full Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity
title_fullStr Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity
title_full_unstemmed Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity
title_short Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity
title_sort antioxidant and neuroprotective activities of hyptis suaveolens (l.) poit. against oxidative stress-induced neurotoxicity
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950622/
https://www.ncbi.nlm.nih.gov/pubmed/24420496
http://dx.doi.org/10.1007/s10571-013-0016-7
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