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Syn5 RNA polymerase synthesizes precise run-off RNA products

The enzyme predominantly used for in vitro run-off RNA synthesis is bacteriophage T7 RNA polymerase. T7 RNA polymerase synthesizes, in addition to run-off products of precise length, transcripts with an additional non-base-paired nucleotide at the 3′-terminus (N + 1 product). This contaminating prod...

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Detalles Bibliográficos
Autores principales:	Zhu, Bin, Tabor, Stanley, Richardson, Charles C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950665/ https://www.ncbi.nlm.nih.gov/pubmed/24285303 http://dx.doi.org/10.1093/nar/gkt1193

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