Structure of the mammalian ribosomal pre-termination complex associated with eRF1•eRF3•GDPNP

Eukaryotic translation termination results from the complex functional interplay between two release factors, eRF1 and eRF3, in which GTP hydrolysis by eRF3 couples codon recognition with peptidyl-tRNA hydrolysis by eRF1. Here, we present a cryo-electron microscopy structure of pre-termination compl...

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Autores principales: des Georges, Amédée, Hashem, Yaser, Unbehaun, Anett, Grassucci, Robert A., Taylor, Derek, Hellen, Christopher U. T., Pestova, Tatyana V., Frank, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950680/
https://www.ncbi.nlm.nih.gov/pubmed/24335085
http://dx.doi.org/10.1093/nar/gkt1279
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author des Georges, Amédée
Hashem, Yaser
Unbehaun, Anett
Grassucci, Robert A.
Taylor, Derek
Hellen, Christopher U. T.
Pestova, Tatyana V.
Frank, Joachim
author_facet des Georges, Amédée
Hashem, Yaser
Unbehaun, Anett
Grassucci, Robert A.
Taylor, Derek
Hellen, Christopher U. T.
Pestova, Tatyana V.
Frank, Joachim
author_sort des Georges, Amédée
collection PubMed
description Eukaryotic translation termination results from the complex functional interplay between two release factors, eRF1 and eRF3, in which GTP hydrolysis by eRF3 couples codon recognition with peptidyl-tRNA hydrolysis by eRF1. Here, we present a cryo-electron microscopy structure of pre-termination complexes associated with eRF1•eRF3•GDPNP at 9.7 -Å resolution, which corresponds to the initial pre-GTP hydrolysis stage of factor attachment and stop codon recognition. It reveals the ribosomal positions of eRFs and provides insights into the mechanisms of stop codon recognition and triggering of eRF3’s GTPase activity.
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spelling pubmed-39506802014-03-12 Structure of the mammalian ribosomal pre-termination complex associated with eRF1•eRF3•GDPNP des Georges, Amédée Hashem, Yaser Unbehaun, Anett Grassucci, Robert A. Taylor, Derek Hellen, Christopher U. T. Pestova, Tatyana V. Frank, Joachim Nucleic Acids Res Eukaryotic translation termination results from the complex functional interplay between two release factors, eRF1 and eRF3, in which GTP hydrolysis by eRF3 couples codon recognition with peptidyl-tRNA hydrolysis by eRF1. Here, we present a cryo-electron microscopy structure of pre-termination complexes associated with eRF1•eRF3•GDPNP at 9.7 -Å resolution, which corresponds to the initial pre-GTP hydrolysis stage of factor attachment and stop codon recognition. It reveals the ribosomal positions of eRFs and provides insights into the mechanisms of stop codon recognition and triggering of eRF3’s GTPase activity. Oxford University Press 2014-03 2013-12-11 /pmc/articles/PMC3950680/ /pubmed/24335085 http://dx.doi.org/10.1093/nar/gkt1279 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle des Georges, Amédée
Hashem, Yaser
Unbehaun, Anett
Grassucci, Robert A.
Taylor, Derek
Hellen, Christopher U. T.
Pestova, Tatyana V.
Frank, Joachim
Structure of the mammalian ribosomal pre-termination complex associated with eRF1•eRF3•GDPNP
title Structure of the mammalian ribosomal pre-termination complex associated with eRF1•eRF3•GDPNP
title_full Structure of the mammalian ribosomal pre-termination complex associated with eRF1•eRF3•GDPNP
title_fullStr Structure of the mammalian ribosomal pre-termination complex associated with eRF1•eRF3•GDPNP
title_full_unstemmed Structure of the mammalian ribosomal pre-termination complex associated with eRF1•eRF3•GDPNP
title_short Structure of the mammalian ribosomal pre-termination complex associated with eRF1•eRF3•GDPNP
title_sort structure of the mammalian ribosomal pre-termination complex associated with erf1•erf3•gdpnp
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950680/
https://www.ncbi.nlm.nih.gov/pubmed/24335085
http://dx.doi.org/10.1093/nar/gkt1279
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