The effects of polymorphisms on human gene targeting

DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targetin...

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Autores principales: Deyle, David R., Li, Li B., Ren, Gaoying, Russell, David W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950700/
https://www.ncbi.nlm.nih.gov/pubmed/24371280
http://dx.doi.org/10.1093/nar/gkt1303
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author Deyle, David R.
Li, Li B.
Ren, Gaoying
Russell, David W.
author_facet Deyle, David R.
Li, Li B.
Ren, Gaoying
Russell, David W.
author_sort Deyle, David R.
collection PubMed
description DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions.
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spelling pubmed-39507002014-03-12 The effects of polymorphisms on human gene targeting Deyle, David R. Li, Li B. Ren, Gaoying Russell, David W. Nucleic Acids Res DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions. Oxford University Press 2014-03 2013-12-25 /pmc/articles/PMC3950700/ /pubmed/24371280 http://dx.doi.org/10.1093/nar/gkt1303 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Deyle, David R.
Li, Li B.
Ren, Gaoying
Russell, David W.
The effects of polymorphisms on human gene targeting
title The effects of polymorphisms on human gene targeting
title_full The effects of polymorphisms on human gene targeting
title_fullStr The effects of polymorphisms on human gene targeting
title_full_unstemmed The effects of polymorphisms on human gene targeting
title_short The effects of polymorphisms on human gene targeting
title_sort effects of polymorphisms on human gene targeting
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950700/
https://www.ncbi.nlm.nih.gov/pubmed/24371280
http://dx.doi.org/10.1093/nar/gkt1303
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