Improved seamless mutagenesis by recombineering using ccdB for counterselection

Recombineering, which is the use of homologous recombination for DNA engineering in Escherichia coli, usually uses antibiotic selection to identify the intended recombinant. When combined in a second step with counterselection using a small molecule toxin, seamless products can be obtained. Here, we...

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Detalles Bibliográficos
Autores principales: Wang, Hailong, Bian, Xiaoying, Xia, Liqiu, Ding, Xuezhi, Müller, Rolf, Zhang, Youming, Fu, Jun, Stewart, A. Francis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950717/
https://www.ncbi.nlm.nih.gov/pubmed/24369425
http://dx.doi.org/10.1093/nar/gkt1339

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950717/
https://www.ncbi.nlm.nih.gov/pubmed/24369425
http://dx.doi.org/10.1093/nar/gkt1339

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