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A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity

In eukaryotic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes is a critical step in post-transcriptional gene regulation. The ribonuclease activity of deadenylase enzymes is attributed to either a DEDD (Asp-Glu-Asp-Asp) or an endonuclease–exonuclease–...

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Detalles Bibliográficos
Autores principales:	Maryati, Maryati, Kaur, Ishwinder, Jadhav, Gopal P., Olotu-Umoren, Loyin, Oveh, Blessing, Hashmi, Lubna, Fischer, Peter M., Winkler, G. Sebastiaan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950723/ https://www.ncbi.nlm.nih.gov/pubmed/24170810 http://dx.doi.org/10.1093/nar/gkt972

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