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Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells
BACKGROUND: Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950800/ https://www.ncbi.nlm.nih.gov/pubmed/24627865 http://dx.doi.org/10.4103/2277-9175.125807 |
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author | Ghodsi, Reihaneh Sadeghi, Hamid Mirmohammad Asghari, Gholamreza Torabi, Sepideh |
author_facet | Ghodsi, Reihaneh Sadeghi, Hamid Mirmohammad Asghari, Gholamreza Torabi, Sepideh |
author_sort | Ghodsi, Reihaneh |
collection | PubMed |
description | BACKGROUND: Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Therefore, the aim of this work was to look for certain water clarification genes in M. peregrina. MATERIALS AND METHODS: After preparation of M. peregrina callus, mRNA was extracted from these cells. After application of reverse transcriptase, the obtained cDNA (s) were used for PCR amplification of the desired genes using primers based on MO(2.1) gene of Moringa oleifera. DNA amplification products were cloned in E. coli Xl(1) blue cells and DNA sequences were compared with Mo(1,2) gene in M. oleifera. RESULTS: We obtained 3 PCR products (approximately 200, 300, and 400 bps). CONCLUSION: After comparison of the sequences of 300bp band obtained from M. peregrina with Mo(1,2) gene in M. oleifera, it seems that 300bp band is a good candidate to investigate regarding its potential flocculent activity. |
format | Online Article Text |
id | pubmed-3950800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-39508002014-03-13 Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells Ghodsi, Reihaneh Sadeghi, Hamid Mirmohammad Asghari, Gholamreza Torabi, Sepideh Adv Biomed Res Original Article BACKGROUND: Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Therefore, the aim of this work was to look for certain water clarification genes in M. peregrina. MATERIALS AND METHODS: After preparation of M. peregrina callus, mRNA was extracted from these cells. After application of reverse transcriptase, the obtained cDNA (s) were used for PCR amplification of the desired genes using primers based on MO(2.1) gene of Moringa oleifera. DNA amplification products were cloned in E. coli Xl(1) blue cells and DNA sequences were compared with Mo(1,2) gene in M. oleifera. RESULTS: We obtained 3 PCR products (approximately 200, 300, and 400 bps). CONCLUSION: After comparison of the sequences of 300bp band obtained from M. peregrina with Mo(1,2) gene in M. oleifera, it seems that 300bp band is a good candidate to investigate regarding its potential flocculent activity. Medknow Publications & Media Pvt Ltd 2014-01-27 /pmc/articles/PMC3950800/ /pubmed/24627865 http://dx.doi.org/10.4103/2277-9175.125807 Text en Copyright: © 2014 Ghodsi. http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Original Article Ghodsi, Reihaneh Sadeghi, Hamid Mirmohammad Asghari, Gholamreza Torabi, Sepideh Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells |
title | Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells |
title_full | Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells |
title_fullStr | Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells |
title_full_unstemmed | Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells |
title_short | Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells |
title_sort | identification and cloning of putative water clarification genes of moringa peregrina (forssk.) fiori in e. coli xl(1) blue cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950800/ https://www.ncbi.nlm.nih.gov/pubmed/24627865 http://dx.doi.org/10.4103/2277-9175.125807 |
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