Efficient Human Breast Cancer Xenograft Regression after a Single Treatment with a Novel Liposomal Formulation of Epirubicin Prepared Using the EDTA Ion Gradient Method

Liposomes act as efficient drug carriers. Recently, epirubicin (EPI) formulation was developed using a novel EDTA ion gradient method for drug encapsulation. This formulation displayed very good stability and drug retention in vitro in a two-year long-term stability experiment. The cryo-TEM images s...

Descripción completa

Detalles Bibliográficos
Autores principales: Gubernator, Jerzy, Lipka, Dominik, Korycińska, Mariola, Kempińska, Katarzyna, Milczarek, Magdalena, Wietrzyk, Joanna, Hrynyk, Rafał, Barnert, Sabine, Süss, Regine, Kozubek, Arkadiusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3951386/
https://www.ncbi.nlm.nih.gov/pubmed/24621591
http://dx.doi.org/10.1371/journal.pone.0091487
_version_ 1782307115979767808
author Gubernator, Jerzy
Lipka, Dominik
Korycińska, Mariola
Kempińska, Katarzyna
Milczarek, Magdalena
Wietrzyk, Joanna
Hrynyk, Rafał
Barnert, Sabine
Süss, Regine
Kozubek, Arkadiusz
author_facet Gubernator, Jerzy
Lipka, Dominik
Korycińska, Mariola
Kempińska, Katarzyna
Milczarek, Magdalena
Wietrzyk, Joanna
Hrynyk, Rafał
Barnert, Sabine
Süss, Regine
Kozubek, Arkadiusz
author_sort Gubernator, Jerzy
collection PubMed
description Liposomes act as efficient drug carriers. Recently, epirubicin (EPI) formulation was developed using a novel EDTA ion gradient method for drug encapsulation. This formulation displayed very good stability and drug retention in vitro in a two-year long-term stability experiment. The cryo-TEM images show drug precipitate structures different than ones formed with ammonium sulfate method, which is usually used to encapsulate anthracyclines. Its pharmacokinetic properties and its efficacy in the human breast MDA-MB-231 cancer xenograft model were also determined. The liposomal EPI formulation is eliminated slowly with an AUC of 7.6487, while the free drug has an AUC of only 0.0097. The formulation also had a much higher overall antitumor efficacy than the free drug.
format Online
Article
Text
id pubmed-3951386
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39513862014-03-13 Efficient Human Breast Cancer Xenograft Regression after a Single Treatment with a Novel Liposomal Formulation of Epirubicin Prepared Using the EDTA Ion Gradient Method Gubernator, Jerzy Lipka, Dominik Korycińska, Mariola Kempińska, Katarzyna Milczarek, Magdalena Wietrzyk, Joanna Hrynyk, Rafał Barnert, Sabine Süss, Regine Kozubek, Arkadiusz PLoS One Research Article Liposomes act as efficient drug carriers. Recently, epirubicin (EPI) formulation was developed using a novel EDTA ion gradient method for drug encapsulation. This formulation displayed very good stability and drug retention in vitro in a two-year long-term stability experiment. The cryo-TEM images show drug precipitate structures different than ones formed with ammonium sulfate method, which is usually used to encapsulate anthracyclines. Its pharmacokinetic properties and its efficacy in the human breast MDA-MB-231 cancer xenograft model were also determined. The liposomal EPI formulation is eliminated slowly with an AUC of 7.6487, while the free drug has an AUC of only 0.0097. The formulation also had a much higher overall antitumor efficacy than the free drug. Public Library of Science 2014-03-12 /pmc/articles/PMC3951386/ /pubmed/24621591 http://dx.doi.org/10.1371/journal.pone.0091487 Text en © 2014 Gubernator et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gubernator, Jerzy
Lipka, Dominik
Korycińska, Mariola
Kempińska, Katarzyna
Milczarek, Magdalena
Wietrzyk, Joanna
Hrynyk, Rafał
Barnert, Sabine
Süss, Regine
Kozubek, Arkadiusz
Efficient Human Breast Cancer Xenograft Regression after a Single Treatment with a Novel Liposomal Formulation of Epirubicin Prepared Using the EDTA Ion Gradient Method
title Efficient Human Breast Cancer Xenograft Regression after a Single Treatment with a Novel Liposomal Formulation of Epirubicin Prepared Using the EDTA Ion Gradient Method
title_full Efficient Human Breast Cancer Xenograft Regression after a Single Treatment with a Novel Liposomal Formulation of Epirubicin Prepared Using the EDTA Ion Gradient Method
title_fullStr Efficient Human Breast Cancer Xenograft Regression after a Single Treatment with a Novel Liposomal Formulation of Epirubicin Prepared Using the EDTA Ion Gradient Method
title_full_unstemmed Efficient Human Breast Cancer Xenograft Regression after a Single Treatment with a Novel Liposomal Formulation of Epirubicin Prepared Using the EDTA Ion Gradient Method
title_short Efficient Human Breast Cancer Xenograft Regression after a Single Treatment with a Novel Liposomal Formulation of Epirubicin Prepared Using the EDTA Ion Gradient Method
title_sort efficient human breast cancer xenograft regression after a single treatment with a novel liposomal formulation of epirubicin prepared using the edta ion gradient method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3951386/
https://www.ncbi.nlm.nih.gov/pubmed/24621591
http://dx.doi.org/10.1371/journal.pone.0091487
work_keys_str_mv AT gubernatorjerzy efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod
AT lipkadominik efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod
AT korycinskamariola efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod
AT kempinskakatarzyna efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod
AT milczarekmagdalena efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod
AT wietrzykjoanna efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod
AT hrynykrafał efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod
AT barnertsabine efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod
AT sussregine efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod
AT kozubekarkadiusz efficienthumanbreastcancerxenograftregressionafterasingletreatmentwithanovelliposomalformulationofepirubicinpreparedusingtheedtaiongradientmethod

Ejemplares similares