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Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration

BACKGROUND AND PURPOSE: This paper evaluated the effect of acute homocysteine administration on inflammatory cytokine tumor necrosis factor-alpha (TNF-α) expression and neuronal apoptosis in the rat hippocampus and investigated the effects of vitamin C treatment on homocysteine-induced inflammation...

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Autores principales: Kim, Seong Hee, Kim, Kwangsoo, Ahn, Ji Hyun, Chang, Hee Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Epilepsy Society 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3952314/
https://www.ncbi.nlm.nih.gov/pubmed/24649438
http://dx.doi.org/10.14581/jer.11002
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author Kim, Seong Hee
Kim, Kwangsoo
Ahn, Ji Hyun
Chang, Hee Kyung
author_facet Kim, Seong Hee
Kim, Kwangsoo
Ahn, Ji Hyun
Chang, Hee Kyung
author_sort Kim, Seong Hee
collection PubMed
description BACKGROUND AND PURPOSE: This paper evaluated the effect of acute homocysteine administration on inflammatory cytokine tumor necrosis factor-alpha (TNF-α) expression and neuronal apoptosis in the rat hippocampus and investigated the effects of vitamin C treatment on homocysteine-induced inflammation and neuronal death. METHODS: Subjects were three-week-old, male Sprague-Dawley rats. Rats for the control group, we injected saline solution into the rats’ abdominal cavities for one week. Rats in the second group received 1 injection of homocysteine (11 mmol/kg) into their abdominal cavities after 1 week of saline solution administration. For the third group, we injected the rats with vitamin C (100 mg/kg) for a week, followed by 1 injection of homocysteine. The hippocampi were stained with an anti-TNF-α antibody, and apoptosis was evaluated using the TUNEL staining method. RESULTS: The homocysteine-injected rats had strong TNF-α expression in every hippocampal region. Vitamin C significantly reduced TNF-α expression in the hippocampus’s CA1 region. Acute homocysteine administration did not cause apoptosis in the hippocampus. CONCLUSIONS: The pro-inflammatory cytokine TNF-α may mediate elevated homocysteine levels’ contributions to inflammatory reactions, and vitamin C has some protective effect on inflammatory reactions in the CA1 hippocampal region.
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spelling pubmed-39523142014-03-19 Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration Kim, Seong Hee Kim, Kwangsoo Ahn, Ji Hyun Chang, Hee Kyung J Epilepsy Res Original Article BACKGROUND AND PURPOSE: This paper evaluated the effect of acute homocysteine administration on inflammatory cytokine tumor necrosis factor-alpha (TNF-α) expression and neuronal apoptosis in the rat hippocampus and investigated the effects of vitamin C treatment on homocysteine-induced inflammation and neuronal death. METHODS: Subjects were three-week-old, male Sprague-Dawley rats. Rats for the control group, we injected saline solution into the rats’ abdominal cavities for one week. Rats in the second group received 1 injection of homocysteine (11 mmol/kg) into their abdominal cavities after 1 week of saline solution administration. For the third group, we injected the rats with vitamin C (100 mg/kg) for a week, followed by 1 injection of homocysteine. The hippocampi were stained with an anti-TNF-α antibody, and apoptosis was evaluated using the TUNEL staining method. RESULTS: The homocysteine-injected rats had strong TNF-α expression in every hippocampal region. Vitamin C significantly reduced TNF-α expression in the hippocampus’s CA1 region. Acute homocysteine administration did not cause apoptosis in the hippocampus. CONCLUSIONS: The pro-inflammatory cytokine TNF-α may mediate elevated homocysteine levels’ contributions to inflammatory reactions, and vitamin C has some protective effect on inflammatory reactions in the CA1 hippocampal region. Korean Epilepsy Society 2011-03-30 /pmc/articles/PMC3952314/ /pubmed/24649438 http://dx.doi.org/10.14581/jer.11002 Text en Copyright © 2011 Korean Epilepsy Society This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Seong Hee
Kim, Kwangsoo
Ahn, Ji Hyun
Chang, Hee Kyung
Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration
title Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration
title_full Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration
title_fullStr Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration
title_full_unstemmed Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration
title_short Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration
title_sort increased expression of tumor necrosis factor-alpha in the rat hippocampus after acute homocysteine administration
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3952314/
https://www.ncbi.nlm.nih.gov/pubmed/24649438
http://dx.doi.org/10.14581/jer.11002
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