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Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration
BACKGROUND AND PURPOSE: This paper evaluated the effect of acute homocysteine administration on inflammatory cytokine tumor necrosis factor-alpha (TNF-α) expression and neuronal apoptosis in the rat hippocampus and investigated the effects of vitamin C treatment on homocysteine-induced inflammation...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Epilepsy Society
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3952314/ https://www.ncbi.nlm.nih.gov/pubmed/24649438 http://dx.doi.org/10.14581/jer.11002 |
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author | Kim, Seong Hee Kim, Kwangsoo Ahn, Ji Hyun Chang, Hee Kyung |
author_facet | Kim, Seong Hee Kim, Kwangsoo Ahn, Ji Hyun Chang, Hee Kyung |
author_sort | Kim, Seong Hee |
collection | PubMed |
description | BACKGROUND AND PURPOSE: This paper evaluated the effect of acute homocysteine administration on inflammatory cytokine tumor necrosis factor-alpha (TNF-α) expression and neuronal apoptosis in the rat hippocampus and investigated the effects of vitamin C treatment on homocysteine-induced inflammation and neuronal death. METHODS: Subjects were three-week-old, male Sprague-Dawley rats. Rats for the control group, we injected saline solution into the rats’ abdominal cavities for one week. Rats in the second group received 1 injection of homocysteine (11 mmol/kg) into their abdominal cavities after 1 week of saline solution administration. For the third group, we injected the rats with vitamin C (100 mg/kg) for a week, followed by 1 injection of homocysteine. The hippocampi were stained with an anti-TNF-α antibody, and apoptosis was evaluated using the TUNEL staining method. RESULTS: The homocysteine-injected rats had strong TNF-α expression in every hippocampal region. Vitamin C significantly reduced TNF-α expression in the hippocampus’s CA1 region. Acute homocysteine administration did not cause apoptosis in the hippocampus. CONCLUSIONS: The pro-inflammatory cytokine TNF-α may mediate elevated homocysteine levels’ contributions to inflammatory reactions, and vitamin C has some protective effect on inflammatory reactions in the CA1 hippocampal region. |
format | Online Article Text |
id | pubmed-3952314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Korean Epilepsy Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-39523142014-03-19 Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration Kim, Seong Hee Kim, Kwangsoo Ahn, Ji Hyun Chang, Hee Kyung J Epilepsy Res Original Article BACKGROUND AND PURPOSE: This paper evaluated the effect of acute homocysteine administration on inflammatory cytokine tumor necrosis factor-alpha (TNF-α) expression and neuronal apoptosis in the rat hippocampus and investigated the effects of vitamin C treatment on homocysteine-induced inflammation and neuronal death. METHODS: Subjects were three-week-old, male Sprague-Dawley rats. Rats for the control group, we injected saline solution into the rats’ abdominal cavities for one week. Rats in the second group received 1 injection of homocysteine (11 mmol/kg) into their abdominal cavities after 1 week of saline solution administration. For the third group, we injected the rats with vitamin C (100 mg/kg) for a week, followed by 1 injection of homocysteine. The hippocampi were stained with an anti-TNF-α antibody, and apoptosis was evaluated using the TUNEL staining method. RESULTS: The homocysteine-injected rats had strong TNF-α expression in every hippocampal region. Vitamin C significantly reduced TNF-α expression in the hippocampus’s CA1 region. Acute homocysteine administration did not cause apoptosis in the hippocampus. CONCLUSIONS: The pro-inflammatory cytokine TNF-α may mediate elevated homocysteine levels’ contributions to inflammatory reactions, and vitamin C has some protective effect on inflammatory reactions in the CA1 hippocampal region. Korean Epilepsy Society 2011-03-30 /pmc/articles/PMC3952314/ /pubmed/24649438 http://dx.doi.org/10.14581/jer.11002 Text en Copyright © 2011 Korean Epilepsy Society This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Seong Hee Kim, Kwangsoo Ahn, Ji Hyun Chang, Hee Kyung Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration |
title | Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration |
title_full | Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration |
title_fullStr | Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration |
title_full_unstemmed | Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration |
title_short | Increased Expression of Tumor Necrosis Factor-Alpha in the Rat Hippocampus after Acute Homocysteine Administration |
title_sort | increased expression of tumor necrosis factor-alpha in the rat hippocampus after acute homocysteine administration |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3952314/ https://www.ncbi.nlm.nih.gov/pubmed/24649438 http://dx.doi.org/10.14581/jer.11002 |
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