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Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells

The microvasculature is important for vertebrate organ development and homeostasis. However, the molecular mechanism of microvascular angiogenesis remains incompletely understood. Through studying Borg5 (Binder of the Rho GTPase 5), which belongs to a family of poorly understood effector proteins of...

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Autores principales: Liu, Zhonghua, Vong, Queenie P., Liu, Chengyu, Zheng, Yixian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3952853/
https://www.ncbi.nlm.nih.gov/pubmed/24451259
http://dx.doi.org/10.1091/mbc.E13-09-0543
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author Liu, Zhonghua
Vong, Queenie P.
Liu, Chengyu
Zheng, Yixian
author_facet Liu, Zhonghua
Vong, Queenie P.
Liu, Chengyu
Zheng, Yixian
author_sort Liu, Zhonghua
collection PubMed
description The microvasculature is important for vertebrate organ development and homeostasis. However, the molecular mechanism of microvascular angiogenesis remains incompletely understood. Through studying Borg5 (Binder of the Rho GTPase 5), which belongs to a family of poorly understood effector proteins of the Cdc42 GTPase, we uncover a role for Borg5 in microvascular angiogenesis. Deletion of Borg5 in mice results in defects in retinal and cardiac microvasculature as well as heart development. Borg5 promotes angiogenesis by regulating persistent directional migration of the endothelial cells (ECs). In primary mouse cardiac ECs (MCECs), Borg5 associates with septins in the perinuclear region and colocalizes with actomyosin fibers. Both Borg5 deletion and septin 7 knockdown lead to a disruption of the perinuclear actomyosin and persistent directional migration. Our findings suggest that Borg5 and septin cytoskeleton spatially control actomyosin activity to ensure persistent directional migration of MCECs and efficient microvascular angiogenesis. Our studies reported here should offer a new avenue to further investigate the functions of Borg5, septin, and actomyosin in the microvasculature in the context of development and disease.
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spelling pubmed-39528532014-05-30 Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells Liu, Zhonghua Vong, Queenie P. Liu, Chengyu Zheng, Yixian Mol Biol Cell Articles The microvasculature is important for vertebrate organ development and homeostasis. However, the molecular mechanism of microvascular angiogenesis remains incompletely understood. Through studying Borg5 (Binder of the Rho GTPase 5), which belongs to a family of poorly understood effector proteins of the Cdc42 GTPase, we uncover a role for Borg5 in microvascular angiogenesis. Deletion of Borg5 in mice results in defects in retinal and cardiac microvasculature as well as heart development. Borg5 promotes angiogenesis by regulating persistent directional migration of the endothelial cells (ECs). In primary mouse cardiac ECs (MCECs), Borg5 associates with septins in the perinuclear region and colocalizes with actomyosin fibers. Both Borg5 deletion and septin 7 knockdown lead to a disruption of the perinuclear actomyosin and persistent directional migration. Our findings suggest that Borg5 and septin cytoskeleton spatially control actomyosin activity to ensure persistent directional migration of MCECs and efficient microvascular angiogenesis. Our studies reported here should offer a new avenue to further investigate the functions of Borg5, septin, and actomyosin in the microvasculature in the context of development and disease. The American Society for Cell Biology 2014-03-15 /pmc/articles/PMC3952853/ /pubmed/24451259 http://dx.doi.org/10.1091/mbc.E13-09-0543 Text en © 2014 Liu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Liu, Zhonghua
Vong, Queenie P.
Liu, Chengyu
Zheng, Yixian
Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells
title Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells
title_full Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells
title_fullStr Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells
title_full_unstemmed Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells
title_short Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells
title_sort borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3952853/
https://www.ncbi.nlm.nih.gov/pubmed/24451259
http://dx.doi.org/10.1091/mbc.E13-09-0543
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