Cargando…

Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue

Various nitric oxide modulators (NO donors - SNP, GSNO, DEA NONOate and scavengers – PTIO, cPTIO) were tested to highlight the role of NO under Cd excess in various ontogenetic stages of chamomile (Matricaria chamomilla). Surprisingly, compared to Cd alone, SNP and PTIO elevated Cd uptake (confirmed...

Descripción completa

Detalles Bibliográficos
Autores principales: Kováčik, Jozef, Babula, Petr, Klejdus, Bořivoj, Hedbavny, Josef, Jarošová, Markéta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953596/
https://www.ncbi.nlm.nih.gov/pubmed/24626462
http://dx.doi.org/10.1371/journal.pone.0091685
_version_ 1782307384548392960
author Kováčik, Jozef
Babula, Petr
Klejdus, Bořivoj
Hedbavny, Josef
Jarošová, Markéta
author_facet Kováčik, Jozef
Babula, Petr
Klejdus, Bořivoj
Hedbavny, Josef
Jarošová, Markéta
author_sort Kováčik, Jozef
collection PubMed
description Various nitric oxide modulators (NO donors - SNP, GSNO, DEA NONOate and scavengers – PTIO, cPTIO) were tested to highlight the role of NO under Cd excess in various ontogenetic stages of chamomile (Matricaria chamomilla). Surprisingly, compared to Cd alone, SNP and PTIO elevated Cd uptake (confirmed also by PhenGreen staining) but depleted glutathione (partially ascorbic acid) and phytochelatins PC(2) and PC(3) in both older plants (cultured hydroponically) and seedlings (cultured in deionised water). Despite these anomalous impacts, fluorescence staining of NO and ROS confirmed predictable assumptions and revealed reciprocal changes (decrease in NO but increase in ROS after PTIO addition and the opposite after SNP application). Subsequent tests using alternative modulators and seedlings confirmed changes to NO and ROS after application of GSNO and DEA NONOate as mentioned above for SNP while cPTIO altered only NO level (depletion). On the contrary to SNP and PTIO, GSNO, DEA NONOate and cPTIO did not elevate Cd content and phytochelatins (PC(2), PC(3)) were rather elevated. These data provide evidence that various NO modulators are useful in terms of NO and ROS manipulation but interactions with intact plants affect metal uptake and must therefore be used with caution. In this view, cPTIO and DEA NONOate revealed the less pronounced side impacts and are recommended as suitable NO scavenger/donor in plant physiological studies under Cd excess.
format Online
Article
Text
id pubmed-3953596
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39535962014-03-18 Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue Kováčik, Jozef Babula, Petr Klejdus, Bořivoj Hedbavny, Josef Jarošová, Markéta PLoS One Research Article Various nitric oxide modulators (NO donors - SNP, GSNO, DEA NONOate and scavengers – PTIO, cPTIO) were tested to highlight the role of NO under Cd excess in various ontogenetic stages of chamomile (Matricaria chamomilla). Surprisingly, compared to Cd alone, SNP and PTIO elevated Cd uptake (confirmed also by PhenGreen staining) but depleted glutathione (partially ascorbic acid) and phytochelatins PC(2) and PC(3) in both older plants (cultured hydroponically) and seedlings (cultured in deionised water). Despite these anomalous impacts, fluorescence staining of NO and ROS confirmed predictable assumptions and revealed reciprocal changes (decrease in NO but increase in ROS after PTIO addition and the opposite after SNP application). Subsequent tests using alternative modulators and seedlings confirmed changes to NO and ROS after application of GSNO and DEA NONOate as mentioned above for SNP while cPTIO altered only NO level (depletion). On the contrary to SNP and PTIO, GSNO, DEA NONOate and cPTIO did not elevate Cd content and phytochelatins (PC(2), PC(3)) were rather elevated. These data provide evidence that various NO modulators are useful in terms of NO and ROS manipulation but interactions with intact plants affect metal uptake and must therefore be used with caution. In this view, cPTIO and DEA NONOate revealed the less pronounced side impacts and are recommended as suitable NO scavenger/donor in plant physiological studies under Cd excess. Public Library of Science 2014-03-13 /pmc/articles/PMC3953596/ /pubmed/24626462 http://dx.doi.org/10.1371/journal.pone.0091685 Text en © 2014 Kováčik et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kováčik, Jozef
Babula, Petr
Klejdus, Bořivoj
Hedbavny, Josef
Jarošová, Markéta
Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue
title Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue
title_full Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue
title_fullStr Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue
title_full_unstemmed Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue
title_short Unexpected Behavior of Some Nitric Oxide Modulators under Cadmium Excess in Plant Tissue
title_sort unexpected behavior of some nitric oxide modulators under cadmium excess in plant tissue
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953596/
https://www.ncbi.nlm.nih.gov/pubmed/24626462
http://dx.doi.org/10.1371/journal.pone.0091685
work_keys_str_mv AT kovacikjozef unexpectedbehaviorofsomenitricoxidemodulatorsundercadmiumexcessinplanttissue
AT babulapetr unexpectedbehaviorofsomenitricoxidemodulatorsundercadmiumexcessinplanttissue
AT klejdusborivoj unexpectedbehaviorofsomenitricoxidemodulatorsundercadmiumexcessinplanttissue
AT hedbavnyjosef unexpectedbehaviorofsomenitricoxidemodulatorsundercadmiumexcessinplanttissue
AT jarosovamarketa unexpectedbehaviorofsomenitricoxidemodulatorsundercadmiumexcessinplanttissue