Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF

Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol...

Descripción completa

Detalles Bibliográficos
Autores principales: Nishikawa, Kahoko, Hashimoto, Masahiro, Itoh, Yoshiyuki, Hiroi, Sadayuki, Kusai, Akihiko, Hirata, Fumihiko, Sakamoto, Toshihisa, Iwaya, Keiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953719/
https://www.ncbi.nlm.nih.gov/pubmed/24649399
http://dx.doi.org/10.1016/j.fob.2014.02.005
Descripción
Sumario:Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m/z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI-SpiralTOF is a powerful tool for clinical screening and estimating fatty liver.

Ejemplares similares