Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF

Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol...

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Autores principales: Nishikawa, Kahoko, Hashimoto, Masahiro, Itoh, Yoshiyuki, Hiroi, Sadayuki, Kusai, Akihiko, Hirata, Fumihiko, Sakamoto, Toshihisa, Iwaya, Keiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953719/
https://www.ncbi.nlm.nih.gov/pubmed/24649399
http://dx.doi.org/10.1016/j.fob.2014.02.005
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author Nishikawa, Kahoko
Hashimoto, Masahiro
Itoh, Yoshiyuki
Hiroi, Sadayuki
Kusai, Akihiko
Hirata, Fumihiko
Sakamoto, Toshihisa
Iwaya, Keiichi
author_facet Nishikawa, Kahoko
Hashimoto, Masahiro
Itoh, Yoshiyuki
Hiroi, Sadayuki
Kusai, Akihiko
Hirata, Fumihiko
Sakamoto, Toshihisa
Iwaya, Keiichi
author_sort Nishikawa, Kahoko
collection PubMed
description Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m/z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI-SpiralTOF is a powerful tool for clinical screening and estimating fatty liver.
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spelling pubmed-39537192014-03-19 Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF Nishikawa, Kahoko Hashimoto, Masahiro Itoh, Yoshiyuki Hiroi, Sadayuki Kusai, Akihiko Hirata, Fumihiko Sakamoto, Toshihisa Iwaya, Keiichi FEBS Open Bio Article Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m/z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI-SpiralTOF is a powerful tool for clinical screening and estimating fatty liver. Elsevier 2014-02-19 /pmc/articles/PMC3953719/ /pubmed/24649399 http://dx.doi.org/10.1016/j.fob.2014.02.005 Text en © 2014 The Authors
spellingShingle Article
Nishikawa, Kahoko
Hashimoto, Masahiro
Itoh, Yoshiyuki
Hiroi, Sadayuki
Kusai, Akihiko
Hirata, Fumihiko
Sakamoto, Toshihisa
Iwaya, Keiichi
Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF
title Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF
title_full Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF
title_fullStr Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF
title_full_unstemmed Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF
title_short Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF
title_sort detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by maldi-spiraltof
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953719/
https://www.ncbi.nlm.nih.gov/pubmed/24649399
http://dx.doi.org/10.1016/j.fob.2014.02.005
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