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Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF
Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953719/ https://www.ncbi.nlm.nih.gov/pubmed/24649399 http://dx.doi.org/10.1016/j.fob.2014.02.005 |
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author | Nishikawa, Kahoko Hashimoto, Masahiro Itoh, Yoshiyuki Hiroi, Sadayuki Kusai, Akihiko Hirata, Fumihiko Sakamoto, Toshihisa Iwaya, Keiichi |
author_facet | Nishikawa, Kahoko Hashimoto, Masahiro Itoh, Yoshiyuki Hiroi, Sadayuki Kusai, Akihiko Hirata, Fumihiko Sakamoto, Toshihisa Iwaya, Keiichi |
author_sort | Nishikawa, Kahoko |
collection | PubMed |
description | Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m/z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI-SpiralTOF is a powerful tool for clinical screening and estimating fatty liver. |
format | Online Article Text |
id | pubmed-3953719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-39537192014-03-19 Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF Nishikawa, Kahoko Hashimoto, Masahiro Itoh, Yoshiyuki Hiroi, Sadayuki Kusai, Akihiko Hirata, Fumihiko Sakamoto, Toshihisa Iwaya, Keiichi FEBS Open Bio Article Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m/z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI-SpiralTOF is a powerful tool for clinical screening and estimating fatty liver. Elsevier 2014-02-19 /pmc/articles/PMC3953719/ /pubmed/24649399 http://dx.doi.org/10.1016/j.fob.2014.02.005 Text en © 2014 The Authors |
spellingShingle | Article Nishikawa, Kahoko Hashimoto, Masahiro Itoh, Yoshiyuki Hiroi, Sadayuki Kusai, Akihiko Hirata, Fumihiko Sakamoto, Toshihisa Iwaya, Keiichi Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF |
title | Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF |
title_full | Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF |
title_fullStr | Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF |
title_full_unstemmed | Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF |
title_short | Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF |
title_sort | detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by maldi-spiraltof |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953719/ https://www.ncbi.nlm.nih.gov/pubmed/24649399 http://dx.doi.org/10.1016/j.fob.2014.02.005 |
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