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Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors
Lignocellulosic hydrolysate (LCH) inhibitors are a large class of bioactive molecules that arise from pretreatment, hydrolysis, and fermentation of plant biomass. These diverse compounds reduce lignocellulosic biofuel yields by inhibiting cellular processes and diverting energy into cellular respons...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954026/ https://www.ncbi.nlm.nih.gov/pubmed/24672514 http://dx.doi.org/10.3389/fmicb.2014.00090 |
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author | Piotrowski, Jeff S. Zhang, Yaoping Bates, Donna M. Keating, David H. Sato, Trey K. Ong, Irene M. Landick, Robert |
author_facet | Piotrowski, Jeff S. Zhang, Yaoping Bates, Donna M. Keating, David H. Sato, Trey K. Ong, Irene M. Landick, Robert |
author_sort | Piotrowski, Jeff S. |
collection | PubMed |
description | Lignocellulosic hydrolysate (LCH) inhibitors are a large class of bioactive molecules that arise from pretreatment, hydrolysis, and fermentation of plant biomass. These diverse compounds reduce lignocellulosic biofuel yields by inhibiting cellular processes and diverting energy into cellular responses. LCH inhibitors present one of the most significant challenges to efficient biofuel production by microbes. Development of new strains that lessen the effects of LCH inhibitors is an economically favorable strategy relative to expensive detoxification methods that also can reduce sugar content in deconstructed biomass. Systems biology analyses and metabolic modeling combined with directed evolution and synthetic biology are successful strategies for biocatalyst development, and methods that leverage state-of-the-art tools are needed to overcome inhibitors more completely. This perspective considers the energetic costs of LCH inhibitors and technologies that can be used to overcome their drain on conversion efficiency. We suggest academic and commercial research groups could benefit by sharing data on LCH inhibitors and implementing “translational biofuel research.” |
format | Online Article Text |
id | pubmed-3954026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-39540262014-03-26 Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors Piotrowski, Jeff S. Zhang, Yaoping Bates, Donna M. Keating, David H. Sato, Trey K. Ong, Irene M. Landick, Robert Front Microbiol Microbiology Lignocellulosic hydrolysate (LCH) inhibitors are a large class of bioactive molecules that arise from pretreatment, hydrolysis, and fermentation of plant biomass. These diverse compounds reduce lignocellulosic biofuel yields by inhibiting cellular processes and diverting energy into cellular responses. LCH inhibitors present one of the most significant challenges to efficient biofuel production by microbes. Development of new strains that lessen the effects of LCH inhibitors is an economically favorable strategy relative to expensive detoxification methods that also can reduce sugar content in deconstructed biomass. Systems biology analyses and metabolic modeling combined with directed evolution and synthetic biology are successful strategies for biocatalyst development, and methods that leverage state-of-the-art tools are needed to overcome inhibitors more completely. This perspective considers the energetic costs of LCH inhibitors and technologies that can be used to overcome their drain on conversion efficiency. We suggest academic and commercial research groups could benefit by sharing data on LCH inhibitors and implementing “translational biofuel research.” Frontiers Media S.A. 2014-03-14 /pmc/articles/PMC3954026/ /pubmed/24672514 http://dx.doi.org/10.3389/fmicb.2014.00090 Text en Copyright © 2014 Piotrowski, Zhang, Bates, Keating, Sato, Ong and Landick. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Piotrowski, Jeff S. Zhang, Yaoping Bates, Donna M. Keating, David H. Sato, Trey K. Ong, Irene M. Landick, Robert Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
title | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
title_full | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
title_fullStr | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
title_full_unstemmed | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
title_short | Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
title_sort | death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954026/ https://www.ncbi.nlm.nih.gov/pubmed/24672514 http://dx.doi.org/10.3389/fmicb.2014.00090 |
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