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Tracking of Magnetite Labeled Nanoparticles in the Rat Brain Using MRI
This study was performed to explore the feasibility of tracing nanoparticles for drug transport in the healthy rat brain with a clinical MRI scanner. Phantom studies were performed to assess the R(1) ( = 1/T(1)) relaxivity of different magnetically labeled nanoparticle (MLNP) formulations that were...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954869/ https://www.ncbi.nlm.nih.gov/pubmed/24633006 http://dx.doi.org/10.1371/journal.pone.0092068 |
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author | Martínez Vera, Naira P. Schmidt, Reinhold Langer, Klaus Zlatev, Iavor Wronski, Robert Auer, Ewald Havas, Daniel Windisch, Manfred von Briesen, Hagen Wagner, Sylvia Stab, Julia Deutsch, Motti Pietrzik, Claus Fazekas, Franz Ropele, Stefan |
author_facet | Martínez Vera, Naira P. Schmidt, Reinhold Langer, Klaus Zlatev, Iavor Wronski, Robert Auer, Ewald Havas, Daniel Windisch, Manfred von Briesen, Hagen Wagner, Sylvia Stab, Julia Deutsch, Motti Pietrzik, Claus Fazekas, Franz Ropele, Stefan |
author_sort | Martínez Vera, Naira P. |
collection | PubMed |
description | This study was performed to explore the feasibility of tracing nanoparticles for drug transport in the healthy rat brain with a clinical MRI scanner. Phantom studies were performed to assess the R(1) ( = 1/T(1)) relaxivity of different magnetically labeled nanoparticle (MLNP) formulations that were based on biodegradable human serum albumin and that were labeled with magnetite of different size. In vivo MRI measurements in 26 rats were done at 3T to study the effect and dynamics of MLNP uptake in the rat brain and body. In the brain, MLNPs induced T(1) changes were quantitatively assessed by T(1) relaxation time mapping in vivo and compared to post-mortem results from fluorescence imaging. Following intravenous injection of MLNPs, a visible MLNP uptake was seen in the liver and spleen while no visual effect was seen in the brain. However a histogram analysis of T(1) changes in the brain demonstrated global and diffuse presence of MLNPs. The magnitude of these T(1) changes scaled with post-mortem fluorescence intensity. This study demonstrates the feasibility of tracking even small amounts of magnetite labeled NPs with a sensitive histogram technique in the brain of a living rodent. |
format | Online Article Text |
id | pubmed-3954869 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39548692014-03-18 Tracking of Magnetite Labeled Nanoparticles in the Rat Brain Using MRI Martínez Vera, Naira P. Schmidt, Reinhold Langer, Klaus Zlatev, Iavor Wronski, Robert Auer, Ewald Havas, Daniel Windisch, Manfred von Briesen, Hagen Wagner, Sylvia Stab, Julia Deutsch, Motti Pietrzik, Claus Fazekas, Franz Ropele, Stefan PLoS One Research Article This study was performed to explore the feasibility of tracing nanoparticles for drug transport in the healthy rat brain with a clinical MRI scanner. Phantom studies were performed to assess the R(1) ( = 1/T(1)) relaxivity of different magnetically labeled nanoparticle (MLNP) formulations that were based on biodegradable human serum albumin and that were labeled with magnetite of different size. In vivo MRI measurements in 26 rats were done at 3T to study the effect and dynamics of MLNP uptake in the rat brain and body. In the brain, MLNPs induced T(1) changes were quantitatively assessed by T(1) relaxation time mapping in vivo and compared to post-mortem results from fluorescence imaging. Following intravenous injection of MLNPs, a visible MLNP uptake was seen in the liver and spleen while no visual effect was seen in the brain. However a histogram analysis of T(1) changes in the brain demonstrated global and diffuse presence of MLNPs. The magnitude of these T(1) changes scaled with post-mortem fluorescence intensity. This study demonstrates the feasibility of tracking even small amounts of magnetite labeled NPs with a sensitive histogram technique in the brain of a living rodent. Public Library of Science 2014-03-14 /pmc/articles/PMC3954869/ /pubmed/24633006 http://dx.doi.org/10.1371/journal.pone.0092068 Text en © 2014 Martínez Vera et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Martínez Vera, Naira P. Schmidt, Reinhold Langer, Klaus Zlatev, Iavor Wronski, Robert Auer, Ewald Havas, Daniel Windisch, Manfred von Briesen, Hagen Wagner, Sylvia Stab, Julia Deutsch, Motti Pietrzik, Claus Fazekas, Franz Ropele, Stefan Tracking of Magnetite Labeled Nanoparticles in the Rat Brain Using MRI |
title | Tracking of Magnetite Labeled Nanoparticles in the Rat Brain Using MRI |
title_full | Tracking of Magnetite Labeled Nanoparticles in the Rat Brain Using MRI |
title_fullStr | Tracking of Magnetite Labeled Nanoparticles in the Rat Brain Using MRI |
title_full_unstemmed | Tracking of Magnetite Labeled Nanoparticles in the Rat Brain Using MRI |
title_short | Tracking of Magnetite Labeled Nanoparticles in the Rat Brain Using MRI |
title_sort | tracking of magnetite labeled nanoparticles in the rat brain using mri |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954869/ https://www.ncbi.nlm.nih.gov/pubmed/24633006 http://dx.doi.org/10.1371/journal.pone.0092068 |
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