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A Novel Chimeric Avidin with Increased Thermal Stability Using DNA Shuffling
Avidins are a family of proteins widely employed in biotechnology. We have previously shown that functional chimeric mutant proteins can be created from avidin and avidin-related protein 2 using a methodology combining random mutagenesis by recombination and selection by a tailored biopanning protoc...
Autores principales: | Taskinen, Barbara, Airenne, Tomi T., Jänis, Janne, Rahikainen, Rolle, Johnson, Mark S., Kulomaa, Markku S., Hytönen, Vesa P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954883/ https://www.ncbi.nlm.nih.gov/pubmed/24632863 http://dx.doi.org/10.1371/journal.pone.0092058 |
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