Evaluation of Paraoxonase Activity in Children With Nephrotic Syndrome

BACKGROUND: It has been proposed that reactive oxygen species (ROS) is involved in the pathogenesis of various diseases. Paraoxonase, a high-density lipoprotein associated enzyme, prevents low-density lipoproteins from oxidation. OBJECTIVES: The aim of the present study was to investigate the serum activities of paraoxonase-1 (PON-1), and aryleterase (ARE) as well as total antioxidant capacity (TAC) in children with nephrotic syndrome in acute and remission phase. PATIENTS AND METHODS: The study consisted of 20 patients in acute and remission phases and 23 healthy controls. PON-1 and ARE activities were determined spectrophotometrically using paraoxone and phenyacetate as substrate, respectively. TAC was measured using ferric reducing ability of plasma (FRAP). RESULTS: The levels of PON, ARE, and TAC were significantly lower in acute phase of nephrotic syndrome compared with the remission phase. The levels of PON, ARE and TAC increased in remission phase. CONCLUSIONS: Our results revealed that the determination of paraoxonase activity might be a biomarker for responses to nephrotic syndrome treatment, which needs to be fully clarified.