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Detection of Toxoplasma gondii from Clinical Specimens of Patients Receiving Renal Transplant Using ELISA and PCR

BACKGROUND: Toxoplasma gondii is an opportunistic parasitic organism causing infection in many mammals, including immunosuppressed patients. Toxoplasmosis as an opportunistic infection is highly prevalent among patients receiving a kidney transplant. OBJECTIVES: The purpose of this study was to iden...

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Autores principales: Izadi, Morteza, Jonaidi Jafari, Nematollah, Mahmoodzadeh Poornaki, Abbas, Sadraei, Javid, Rezavand, Babak, Mirzaei, Hamid Reza, Zarrinfar, Hossein, Khedive, Abulfazl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955291/
https://www.ncbi.nlm.nih.gov/pubmed/24693506
http://dx.doi.org/10.5812/numonthly.13657
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author Izadi, Morteza
Jonaidi Jafari, Nematollah
Mahmoodzadeh Poornaki, Abbas
Sadraei, Javid
Rezavand, Babak
Mirzaei, Hamid Reza
Zarrinfar, Hossein
Khedive, Abulfazl
author_facet Izadi, Morteza
Jonaidi Jafari, Nematollah
Mahmoodzadeh Poornaki, Abbas
Sadraei, Javid
Rezavand, Babak
Mirzaei, Hamid Reza
Zarrinfar, Hossein
Khedive, Abulfazl
author_sort Izadi, Morteza
collection PubMed
description BACKGROUND: Toxoplasma gondii is an opportunistic parasitic organism causing infection in many mammals, including immunosuppressed patients. Toxoplasmosis as an opportunistic infection is highly prevalent among patients receiving a kidney transplant. OBJECTIVES: The purpose of this study was to identify and determine the prevalence of Toxoplasma gondii in clinical samples collected from patients receiving renal transplants. PATIENTS AND METHODS: A total of 50 blood samples and 40 lung lavage samples from transplanted patients admitted to the infectious wards and the patients undergoing bronchoscopy were collected. The B1 Gene of Toxoplasma gondii was amplified using PCR of the blood and bronoalveolar lavage BAL samples, and IgG and IgM antibodies against Toxoplasma were detected in serum samples using ELISA. RESULTS: Our results indicated that anti-toxoplasma specific IgG and IgM antibodies were prevalent among transplant recipients with values of 54% and 4% respectively. PCR was performed to detect Toxoplasma gondii in 3 blood and lavage samples (3.3%) with 100% sensitivity and 97.9% specificity. CONCLUSIONS: Toxoplasma gondii pulmonary infection is measured along with brain toxoplasmosis in patients receiving a kidney transplant. After serological methods, PCR is the second useful method for Toxoplasma gondii screening. Proper prophylaxis before and after receiving a kidney transplant together with Toxoplasma gondii screening of donor and transplant is recommended.
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spelling pubmed-39552912014-04-01 Detection of Toxoplasma gondii from Clinical Specimens of Patients Receiving Renal Transplant Using ELISA and PCR Izadi, Morteza Jonaidi Jafari, Nematollah Mahmoodzadeh Poornaki, Abbas Sadraei, Javid Rezavand, Babak Mirzaei, Hamid Reza Zarrinfar, Hossein Khedive, Abulfazl Nephrourol Mon Research Article BACKGROUND: Toxoplasma gondii is an opportunistic parasitic organism causing infection in many mammals, including immunosuppressed patients. Toxoplasmosis as an opportunistic infection is highly prevalent among patients receiving a kidney transplant. OBJECTIVES: The purpose of this study was to identify and determine the prevalence of Toxoplasma gondii in clinical samples collected from patients receiving renal transplants. PATIENTS AND METHODS: A total of 50 blood samples and 40 lung lavage samples from transplanted patients admitted to the infectious wards and the patients undergoing bronchoscopy were collected. The B1 Gene of Toxoplasma gondii was amplified using PCR of the blood and bronoalveolar lavage BAL samples, and IgG and IgM antibodies against Toxoplasma were detected in serum samples using ELISA. RESULTS: Our results indicated that anti-toxoplasma specific IgG and IgM antibodies were prevalent among transplant recipients with values of 54% and 4% respectively. PCR was performed to detect Toxoplasma gondii in 3 blood and lavage samples (3.3%) with 100% sensitivity and 97.9% specificity. CONCLUSIONS: Toxoplasma gondii pulmonary infection is measured along with brain toxoplasmosis in patients receiving a kidney transplant. After serological methods, PCR is the second useful method for Toxoplasma gondii screening. Proper prophylaxis before and after receiving a kidney transplant together with Toxoplasma gondii screening of donor and transplant is recommended. Kowsar 2013-11-13 2013-11 /pmc/articles/PMC3955291/ /pubmed/24693506 http://dx.doi.org/10.5812/numonthly.13657 Text en Copyright © 2013, Nephrology and Urology Research Center; Published by Kowsar Corp. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Izadi, Morteza
Jonaidi Jafari, Nematollah
Mahmoodzadeh Poornaki, Abbas
Sadraei, Javid
Rezavand, Babak
Mirzaei, Hamid Reza
Zarrinfar, Hossein
Khedive, Abulfazl
Detection of Toxoplasma gondii from Clinical Specimens of Patients Receiving Renal Transplant Using ELISA and PCR
title Detection of Toxoplasma gondii from Clinical Specimens of Patients Receiving Renal Transplant Using ELISA and PCR
title_full Detection of Toxoplasma gondii from Clinical Specimens of Patients Receiving Renal Transplant Using ELISA and PCR
title_fullStr Detection of Toxoplasma gondii from Clinical Specimens of Patients Receiving Renal Transplant Using ELISA and PCR
title_full_unstemmed Detection of Toxoplasma gondii from Clinical Specimens of Patients Receiving Renal Transplant Using ELISA and PCR
title_short Detection of Toxoplasma gondii from Clinical Specimens of Patients Receiving Renal Transplant Using ELISA and PCR
title_sort detection of toxoplasma gondii from clinical specimens of patients receiving renal transplant using elisa and pcr
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955291/
https://www.ncbi.nlm.nih.gov/pubmed/24693506
http://dx.doi.org/10.5812/numonthly.13657
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