(1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii

Almost complete sequence specific (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii are reported. The cDNA of S114A mutant of UVI31+ was cloned from a eukaryotic green algae (C. reinhardtii) and overexpressed in E.coli from where the protein was pur...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Himanshu, Raghavan, Vandana, Shukla, Manish, Rao, Basuthkar J., Chary, Kandala V. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955482/
https://www.ncbi.nlm.nih.gov/pubmed/23266947
http://dx.doi.org/10.1007/s12104-012-9455-1
Descripción
Sumario:Almost complete sequence specific (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii are reported. The cDNA of S114A mutant of UVI31+ was cloned from a eukaryotic green algae (C. reinhardtii) and overexpressed in E.coli from where the protein was purified to homogeneity. The point mutation S114A in UVI31+ reduces its DNA endonuclease activity substantially as compared with its wild type. As a prelude to the structural characterization of S114A mutant of UVI31+, we report here complete sequence-specific (1)H, (13)C and (15)N NMR assignments.

Ejemplares similares