(1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii

Almost complete sequence specific (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii are reported. The cDNA of S114A mutant of UVI31+ was cloned from a eukaryotic green algae (C. reinhardtii) and overexpressed in E.coli from where the protein was pur...

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Autores principales: Singh, Himanshu, Raghavan, Vandana, Shukla, Manish, Rao, Basuthkar J., Chary, Kandala V. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955482/
https://www.ncbi.nlm.nih.gov/pubmed/23266947
http://dx.doi.org/10.1007/s12104-012-9455-1
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author Singh, Himanshu
Raghavan, Vandana
Shukla, Manish
Rao, Basuthkar J.
Chary, Kandala V. R.
author_facet Singh, Himanshu
Raghavan, Vandana
Shukla, Manish
Rao, Basuthkar J.
Chary, Kandala V. R.
author_sort Singh, Himanshu
collection PubMed
description Almost complete sequence specific (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii are reported. The cDNA of S114A mutant of UVI31+ was cloned from a eukaryotic green algae (C. reinhardtii) and overexpressed in E.coli from where the protein was purified to homogeneity. The point mutation S114A in UVI31+ reduces its DNA endonuclease activity substantially as compared with its wild type. As a prelude to the structural characterization of S114A mutant of UVI31+, we report here complete sequence-specific (1)H, (13)C and (15)N NMR assignments.
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spelling pubmed-39554822014-03-21 (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii Singh, Himanshu Raghavan, Vandana Shukla, Manish Rao, Basuthkar J. Chary, Kandala V. R. Biomol NMR Assign Article Almost complete sequence specific (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii are reported. The cDNA of S114A mutant of UVI31+ was cloned from a eukaryotic green algae (C. reinhardtii) and overexpressed in E.coli from where the protein was purified to homogeneity. The point mutation S114A in UVI31+ reduces its DNA endonuclease activity substantially as compared with its wild type. As a prelude to the structural characterization of S114A mutant of UVI31+, we report here complete sequence-specific (1)H, (13)C and (15)N NMR assignments. Springer Netherlands 2012-12-25 2014 /pmc/articles/PMC3955482/ /pubmed/23266947 http://dx.doi.org/10.1007/s12104-012-9455-1 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Singh, Himanshu
Raghavan, Vandana
Shukla, Manish
Rao, Basuthkar J.
Chary, Kandala V. R.
(1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii
title (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii
title_full (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii
title_fullStr (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii
title_full_unstemmed (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii
title_short (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii
title_sort (1)h, (13)c and (15)n resonance assignments of s114a mutant of uvi31+ from chlamydomonas reinhardtii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955482/
https://www.ncbi.nlm.nih.gov/pubmed/23266947
http://dx.doi.org/10.1007/s12104-012-9455-1
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