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Characterization of integrons among Escherichia coli in a region at high incidence of ESBL-EC
Objective : The aim of study was to investigate the distribution of the integrons in Escherichia coli (E. coli) isolates, and analyze the possible relationship between the antimicrobial resistance profiles and the integrons. Methods : The antimicrobial profiles of 376 E. coli strains were analysed b...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Professional Medical Publicaitons
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955567/ https://www.ncbi.nlm.nih.gov/pubmed/24639856 http://dx.doi.org/10.12669/pjms.301.4079 |
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author | Li, Lu-Ming Wang, Ming-Yi Yuan, Xiao-Yan Wang, Hong-Jun Li, Qin Zhu, YA-Mei |
author_facet | Li, Lu-Ming Wang, Ming-Yi Yuan, Xiao-Yan Wang, Hong-Jun Li, Qin Zhu, YA-Mei |
author_sort | Li, Lu-Ming |
collection | PubMed |
description | Objective : The aim of study was to investigate the distribution of the integrons in Escherichia coli (E. coli) isolates, and analyze the possible relationship between the antimicrobial resistance profiles and the integrons. Methods : The antimicrobial profiles of 376 E. coli strains were analysed by disk diffusion test. The integron genes and variable regions were detected by PCR. Some amplicons were sequenced to determine the gene cassettes style. Results : Of 376 isolates, 223 isolates (59.3%) were confirmed as ESBL-EC. Comparison to ESBL-negative E. coli, the high rates of resistance to the third and fourth generation of cephalosporins, penicillins and amikacin were found in ESBL-EC. Only class 1 was integron detected in the isolates, and the prevalence of it was 66.5%. It was commonly found in ESBL-EC (77.6%, 173/223), which was higher than that of ESBL-negative E. coli (50.3%, 77/153) (p<0.001). Six different genes cassettes were detected in this study and were classified into three groups: dfr17-aadA5, dfrA12-aadA2 and aacA4-CmlA1. Additionally, more than one gene array harboured in 13.9% isolates of ESBL-EC, while in 9.1% isolates of ESBL-negative E.coli. Conclusion : The high incidence of ESBL-EC with resistance to multiple antibiotics were detected in the isolates from Blood stream infection (BSI). More resistant gene cassettes in ESBL-EC may partially underlie the high resistance to amikacin, while no relation exists between the high incidence of ESBL-EC and classes 1~ 3 integrons in this region. |
format | Online Article Text |
id | pubmed-3955567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Professional Medical Publicaitons |
record_format | MEDLINE/PubMed |
spelling | pubmed-39555672014-03-17 Characterization of integrons among Escherichia coli in a region at high incidence of ESBL-EC Li, Lu-Ming Wang, Ming-Yi Yuan, Xiao-Yan Wang, Hong-Jun Li, Qin Zhu, YA-Mei Pak J Med Sci Original Article Objective : The aim of study was to investigate the distribution of the integrons in Escherichia coli (E. coli) isolates, and analyze the possible relationship between the antimicrobial resistance profiles and the integrons. Methods : The antimicrobial profiles of 376 E. coli strains were analysed by disk diffusion test. The integron genes and variable regions were detected by PCR. Some amplicons were sequenced to determine the gene cassettes style. Results : Of 376 isolates, 223 isolates (59.3%) were confirmed as ESBL-EC. Comparison to ESBL-negative E. coli, the high rates of resistance to the third and fourth generation of cephalosporins, penicillins and amikacin were found in ESBL-EC. Only class 1 was integron detected in the isolates, and the prevalence of it was 66.5%. It was commonly found in ESBL-EC (77.6%, 173/223), which was higher than that of ESBL-negative E. coli (50.3%, 77/153) (p<0.001). Six different genes cassettes were detected in this study and were classified into three groups: dfr17-aadA5, dfrA12-aadA2 and aacA4-CmlA1. Additionally, more than one gene array harboured in 13.9% isolates of ESBL-EC, while in 9.1% isolates of ESBL-negative E.coli. Conclusion : The high incidence of ESBL-EC with resistance to multiple antibiotics were detected in the isolates from Blood stream infection (BSI). More resistant gene cassettes in ESBL-EC may partially underlie the high resistance to amikacin, while no relation exists between the high incidence of ESBL-EC and classes 1~ 3 integrons in this region. Professional Medical Publicaitons 2014 /pmc/articles/PMC3955567/ /pubmed/24639856 http://dx.doi.org/10.12669/pjms.301.4079 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Li, Lu-Ming Wang, Ming-Yi Yuan, Xiao-Yan Wang, Hong-Jun Li, Qin Zhu, YA-Mei Characterization of integrons among Escherichia coli in a region at high incidence of ESBL-EC |
title | Characterization of integrons among Escherichia coli in a region at high incidence of ESBL-EC |
title_full | Characterization of integrons among Escherichia coli in a region at high incidence of ESBL-EC |
title_fullStr | Characterization of integrons among Escherichia coli in a region at high incidence of ESBL-EC |
title_full_unstemmed | Characterization of integrons among Escherichia coli in a region at high incidence of ESBL-EC |
title_short | Characterization of integrons among Escherichia coli in a region at high incidence of ESBL-EC |
title_sort | characterization of integrons among escherichia coli in a region at high incidence of esbl-ec |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955567/ https://www.ncbi.nlm.nih.gov/pubmed/24639856 http://dx.doi.org/10.12669/pjms.301.4079 |
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