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5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils
The roles of eosinophils in antimicrobial defense remain incompletely understood. In ovalbumin-sensitized mice, eosinophils are selectively recruited to the peritoneal cavity by antigen, eotaxin, or leukotriene(LT)B4, a 5-lipoxygenase (5-LO) metabolite. 5-LO blockade prevents responses to both antig...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955596/ https://www.ncbi.nlm.nih.gov/pubmed/24723744 http://dx.doi.org/10.1155/2014/102160 |
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author | Luz, Ricardo Alves Xavier-Elsas, Pedro de Luca, Bianca Masid-de-Brito, Daniela Cauduro, Priscila Soares Gondar Arcanjo, Luiz Carlos Cordeiro Faria dos Santos, Ana Carolina de Oliveira, Ivi Cristina Maria Gaspar-Elsas, Maria Ignez Capella |
author_facet | Luz, Ricardo Alves Xavier-Elsas, Pedro de Luca, Bianca Masid-de-Brito, Daniela Cauduro, Priscila Soares Gondar Arcanjo, Luiz Carlos Cordeiro Faria dos Santos, Ana Carolina de Oliveira, Ivi Cristina Maria Gaspar-Elsas, Maria Ignez Capella |
author_sort | Luz, Ricardo Alves |
collection | PubMed |
description | The roles of eosinophils in antimicrobial defense remain incompletely understood. In ovalbumin-sensitized mice, eosinophils are selectively recruited to the peritoneal cavity by antigen, eotaxin, or leukotriene(LT)B4, a 5-lipoxygenase (5-LO) metabolite. 5-LO blockade prevents responses to both antigen and eotaxin. We examined responses to eotaxin in the absence of sensitization and their dependence on 5-LO. BALB/c or PAS mice and their mutants (5-LO-deficient ALOX; eosinophil-deficient GATA-1) were injected i.p. with eotaxin, eosinophils, or both, and leukocyte accumulation was quantified up to 24 h. Significant recruitment of eosinophils by eotaxin in BALB/c, up to 24 h, was accompanied by much larger numbers of recruited neutrophils and monocytes/macrophages. These effects were abolished by eotaxin neutralization and 5-LO-activating protein inhibitor MK886. In ALOX (but not PAS) mice, eotaxin recruitment was abolished for eosinophils and halved for neutrophils. In GATA-1 mutants, eotaxin recruited neither neutrophils nor macrophages. Transfer of eosinophils cultured from bone-marrow of BALB/c donors, or from ALOX donors, into GATA-1 mutant recipients, i.p., restored eotaxin recruitment of neutrophils and showed that the critical step dependent on 5-LO is the initial recruitment of eosinophils by eotaxin, not the secondary neutrophil accumulation. Eosinophil-dependent recruitment of neutrophils in naive BALB/c mice was associated with increased binding of bacteria. |
format | Online Article Text |
id | pubmed-3955596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39555962014-04-10 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils Luz, Ricardo Alves Xavier-Elsas, Pedro de Luca, Bianca Masid-de-Brito, Daniela Cauduro, Priscila Soares Gondar Arcanjo, Luiz Carlos Cordeiro Faria dos Santos, Ana Carolina de Oliveira, Ivi Cristina Maria Gaspar-Elsas, Maria Ignez Capella Mediators Inflamm Research Article The roles of eosinophils in antimicrobial defense remain incompletely understood. In ovalbumin-sensitized mice, eosinophils are selectively recruited to the peritoneal cavity by antigen, eotaxin, or leukotriene(LT)B4, a 5-lipoxygenase (5-LO) metabolite. 5-LO blockade prevents responses to both antigen and eotaxin. We examined responses to eotaxin in the absence of sensitization and their dependence on 5-LO. BALB/c or PAS mice and their mutants (5-LO-deficient ALOX; eosinophil-deficient GATA-1) were injected i.p. with eotaxin, eosinophils, or both, and leukocyte accumulation was quantified up to 24 h. Significant recruitment of eosinophils by eotaxin in BALB/c, up to 24 h, was accompanied by much larger numbers of recruited neutrophils and monocytes/macrophages. These effects were abolished by eotaxin neutralization and 5-LO-activating protein inhibitor MK886. In ALOX (but not PAS) mice, eotaxin recruitment was abolished for eosinophils and halved for neutrophils. In GATA-1 mutants, eotaxin recruited neither neutrophils nor macrophages. Transfer of eosinophils cultured from bone-marrow of BALB/c donors, or from ALOX donors, into GATA-1 mutant recipients, i.p., restored eotaxin recruitment of neutrophils and showed that the critical step dependent on 5-LO is the initial recruitment of eosinophils by eotaxin, not the secondary neutrophil accumulation. Eosinophil-dependent recruitment of neutrophils in naive BALB/c mice was associated with increased binding of bacteria. Hindawi Publishing Corporation 2014 2014-02-25 /pmc/articles/PMC3955596/ /pubmed/24723744 http://dx.doi.org/10.1155/2014/102160 Text en Copyright © 2014 Ricardo Alves Luz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Luz, Ricardo Alves Xavier-Elsas, Pedro de Luca, Bianca Masid-de-Brito, Daniela Cauduro, Priscila Soares Gondar Arcanjo, Luiz Carlos Cordeiro Faria dos Santos, Ana Carolina de Oliveira, Ivi Cristina Maria Gaspar-Elsas, Maria Ignez Capella 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
title | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
title_full | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
title_fullStr | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
title_full_unstemmed | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
title_short | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
title_sort | 5-lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955596/ https://www.ncbi.nlm.nih.gov/pubmed/24723744 http://dx.doi.org/10.1155/2014/102160 |
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