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Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing

Terminalia chebula is one of the traditional medicines used in the treatment of many diseases. In the present work, different concentrations of various organic and aqueous extracts (solvent-free) of T. chebula were tested on fibroblast (L929) and keratinocytes cells to evaluate its biocompatible con...

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Detalles Bibliográficos
Autores principales: Singh, Dolly, Singh, Deepti, Choi, Soon Mo, Zo, Sun Mi, Painuli, Rakesh Mohan, Kwon, Sung Won, Han, Sung Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955618/
https://www.ncbi.nlm.nih.gov/pubmed/24719644
http://dx.doi.org/10.1155/2014/701656
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author Singh, Dolly
Singh, Deepti
Choi, Soon Mo
Zo, Sun Mi
Painuli, Rakesh Mohan
Kwon, Sung Won
Han, Sung Soo
author_facet Singh, Dolly
Singh, Deepti
Choi, Soon Mo
Zo, Sun Mi
Painuli, Rakesh Mohan
Kwon, Sung Won
Han, Sung Soo
author_sort Singh, Dolly
collection PubMed
description Terminalia chebula is one of the traditional medicines used in the treatment of many diseases. In the present work, different concentrations of various organic and aqueous extracts (solvent-free) of T. chebula were tested on fibroblast (L929) and keratinocytes cells to evaluate its biocompatible concentration by using MTT and live-dead viability/cytotoxic assay. These extracts were found to be effective in decreasing the ammonia accumulation in the media, thereby reducing its toxic effect on cells. DPPH assay further confirmed the free-radical scavenging ability of the extracts which increased with the increase in concentration of each extract. Cell proliferation/apoptosis, cytoskeletal structure, and ECM production were further evaluated by live-dead assay and phalloidin/cytokeratin staining, respectively. The cytoskeletal structure and ECM secretion of the cells treated with extracts showed higher cellular activity in comparison to control. In conclusion, we have demonstrated the effect of these extracts of T. chebula on both types of skin cells and optimized concentration in which it could be used as a bioactive component for wound healing applications by increasing cell proliferation and decreasing free-radical production without affecting the normal cellular matrix. It can also find applications in other therapeutics applications where ammonia toxicity is a limiting factor.
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spelling pubmed-39556182014-04-09 Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing Singh, Dolly Singh, Deepti Choi, Soon Mo Zo, Sun Mi Painuli, Rakesh Mohan Kwon, Sung Won Han, Sung Soo Evid Based Complement Alternat Med Research Article Terminalia chebula is one of the traditional medicines used in the treatment of many diseases. In the present work, different concentrations of various organic and aqueous extracts (solvent-free) of T. chebula were tested on fibroblast (L929) and keratinocytes cells to evaluate its biocompatible concentration by using MTT and live-dead viability/cytotoxic assay. These extracts were found to be effective in decreasing the ammonia accumulation in the media, thereby reducing its toxic effect on cells. DPPH assay further confirmed the free-radical scavenging ability of the extracts which increased with the increase in concentration of each extract. Cell proliferation/apoptosis, cytoskeletal structure, and ECM production were further evaluated by live-dead assay and phalloidin/cytokeratin staining, respectively. The cytoskeletal structure and ECM secretion of the cells treated with extracts showed higher cellular activity in comparison to control. In conclusion, we have demonstrated the effect of these extracts of T. chebula on both types of skin cells and optimized concentration in which it could be used as a bioactive component for wound healing applications by increasing cell proliferation and decreasing free-radical production without affecting the normal cellular matrix. It can also find applications in other therapeutics applications where ammonia toxicity is a limiting factor. Hindawi Publishing Corporation 2014 2014-02-26 /pmc/articles/PMC3955618/ /pubmed/24719644 http://dx.doi.org/10.1155/2014/701656 Text en Copyright © 2014 Dolly Singh et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Singh, Dolly
Singh, Deepti
Choi, Soon Mo
Zo, Sun Mi
Painuli, Rakesh Mohan
Kwon, Sung Won
Han, Sung Soo
Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing
title Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing
title_full Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing
title_fullStr Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing
title_full_unstemmed Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing
title_short Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing
title_sort effect of extracts of terminalia chebula on proliferation of keratinocytes and fibroblasts cells: an alternative approach for wound healing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955618/
https://www.ncbi.nlm.nih.gov/pubmed/24719644
http://dx.doi.org/10.1155/2014/701656
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