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Tissue Factor and Tissue Factor Pathway Inhibitor in Chronically Inflamed Gallbladder Mucosa

We characterised a tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression in relation to severity of inflammatory infiltration of the gallbladder mucosa in a chronic cholecystitis. We prospectively studied the gallbladder specimens obtained from 54 patients who had undergone cholec...

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Autores principales: Liczko, Jacek, Stawski, Tomasz, Żaba, Małgorzata, Kurek, Józef, Sabat, Daniel, Wyrobiec, Grzegorz, Domal-Kwiatkowska, Dorota, Dudek, Damian, Kucharzewski, Marek, Helewski, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955627/
https://www.ncbi.nlm.nih.gov/pubmed/24716194
http://dx.doi.org/10.1155/2014/403639
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author Liczko, Jacek
Stawski, Tomasz
Żaba, Małgorzata
Kurek, Józef
Sabat, Daniel
Wyrobiec, Grzegorz
Domal-Kwiatkowska, Dorota
Dudek, Damian
Kucharzewski, Marek
Helewski, Krzysztof
author_facet Liczko, Jacek
Stawski, Tomasz
Żaba, Małgorzata
Kurek, Józef
Sabat, Daniel
Wyrobiec, Grzegorz
Domal-Kwiatkowska, Dorota
Dudek, Damian
Kucharzewski, Marek
Helewski, Krzysztof
author_sort Liczko, Jacek
collection PubMed
description We characterised a tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression in relation to severity of inflammatory infiltration of the gallbladder mucosa in a chronic cholecystitis. We prospectively studied the gallbladder specimens obtained from 54 patients who had undergone cholecystectomy due to chronic calculous cholecystitis and 16 calculosis-free gallbladder specimens obtained from patients who underwent cholecystectomy due to the polyp/polyps as well as in cases of gallbladder injury. To assess TF and TFPI immunoreactivity by immunohistochemistry, the monoclonal anti-human TF and TFPI antibodies were used. The inflammatory infiltration of the gallbladder mucosa was reflected by the number of CD3 and CD68 positive cells. The expression of TF and TFPI differed significantly between the cholecystitis and the control group. Most capillary endothelial cells of the cholecystitis group presented weak expression for TFPI. The mean number of CD3 positive lymphocytes in the cholecystitis group was 18.6 ± 12.2, but the mean number of CD68 positive cells was 29.7 ± 13.9. In the control sections, it was 3.1 ± 1.9 and 8.8 ± 3.9, respectively (P < 0.001). The results of the current study suggest that the tissue procoagulant state found may be engaged in the etiopathogenesis of the cholecystitis.
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spelling pubmed-39556272014-04-08 Tissue Factor and Tissue Factor Pathway Inhibitor in Chronically Inflamed Gallbladder Mucosa Liczko, Jacek Stawski, Tomasz Żaba, Małgorzata Kurek, Józef Sabat, Daniel Wyrobiec, Grzegorz Domal-Kwiatkowska, Dorota Dudek, Damian Kucharzewski, Marek Helewski, Krzysztof Biomed Res Int Research Article We characterised a tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression in relation to severity of inflammatory infiltration of the gallbladder mucosa in a chronic cholecystitis. We prospectively studied the gallbladder specimens obtained from 54 patients who had undergone cholecystectomy due to chronic calculous cholecystitis and 16 calculosis-free gallbladder specimens obtained from patients who underwent cholecystectomy due to the polyp/polyps as well as in cases of gallbladder injury. To assess TF and TFPI immunoreactivity by immunohistochemistry, the monoclonal anti-human TF and TFPI antibodies were used. The inflammatory infiltration of the gallbladder mucosa was reflected by the number of CD3 and CD68 positive cells. The expression of TF and TFPI differed significantly between the cholecystitis and the control group. Most capillary endothelial cells of the cholecystitis group presented weak expression for TFPI. The mean number of CD3 positive lymphocytes in the cholecystitis group was 18.6 ± 12.2, but the mean number of CD68 positive cells was 29.7 ± 13.9. In the control sections, it was 3.1 ± 1.9 and 8.8 ± 3.9, respectively (P < 0.001). The results of the current study suggest that the tissue procoagulant state found may be engaged in the etiopathogenesis of the cholecystitis. Hindawi Publishing Corporation 2014 2014-02-27 /pmc/articles/PMC3955627/ /pubmed/24716194 http://dx.doi.org/10.1155/2014/403639 Text en Copyright © 2014 Jacek Liczko et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liczko, Jacek
Stawski, Tomasz
Żaba, Małgorzata
Kurek, Józef
Sabat, Daniel
Wyrobiec, Grzegorz
Domal-Kwiatkowska, Dorota
Dudek, Damian
Kucharzewski, Marek
Helewski, Krzysztof
Tissue Factor and Tissue Factor Pathway Inhibitor in Chronically Inflamed Gallbladder Mucosa
title Tissue Factor and Tissue Factor Pathway Inhibitor in Chronically Inflamed Gallbladder Mucosa
title_full Tissue Factor and Tissue Factor Pathway Inhibitor in Chronically Inflamed Gallbladder Mucosa
title_fullStr Tissue Factor and Tissue Factor Pathway Inhibitor in Chronically Inflamed Gallbladder Mucosa
title_full_unstemmed Tissue Factor and Tissue Factor Pathway Inhibitor in Chronically Inflamed Gallbladder Mucosa
title_short Tissue Factor and Tissue Factor Pathway Inhibitor in Chronically Inflamed Gallbladder Mucosa
title_sort tissue factor and tissue factor pathway inhibitor in chronically inflamed gallbladder mucosa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955627/
https://www.ncbi.nlm.nih.gov/pubmed/24716194
http://dx.doi.org/10.1155/2014/403639
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