Cargando…
Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes
Verbascoside (VB) is a bioactive polyphenol from olive oil mill wastewater with known antioxidant activity. Oxidative stress is an emerging problem in assisted reproductive technology (ART). Juvenile ART is a promising topic because, in farm animals, it reduces the generation gap and, in human repro...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955694/ https://www.ncbi.nlm.nih.gov/pubmed/24719893 http://dx.doi.org/10.1155/2014/878062 |
_version_ | 1782307616287883264 |
---|---|
author | Dell'Aquila, M. E. Bogliolo, L. Russo, R. Martino, N. A. Filioli Uranio, M. Ariu, F. Amati, F. Sardanelli, A. M. Linsalata, V. Ferruzzi, M. G. Cardinali, A. Minervini, F. |
author_facet | Dell'Aquila, M. E. Bogliolo, L. Russo, R. Martino, N. A. Filioli Uranio, M. Ariu, F. Amati, F. Sardanelli, A. M. Linsalata, V. Ferruzzi, M. G. Cardinali, A. Minervini, F. |
author_sort | Dell'Aquila, M. E. |
collection | PubMed |
description | Verbascoside (VB) is a bioactive polyphenol from olive oil mill wastewater with known antioxidant activity. Oxidative stress is an emerging problem in assisted reproductive technology (ART). Juvenile ART is a promising topic because, in farm animals, it reduces the generation gap and, in human reproductive medicine, it helps to overcome premature ovarian failure. The aim of this study was to test the effects of VB on the developmental competence of ovine prepubertal oocytes and the bioenergetic/oxidative stress status of fresh and vitrified oocytes. In fresh oocytes, VB exerted prooxidant short-term effects, that is, catalase activity increase and uncoupled increases of mitochondria and reactive oxygen species (ROS) fluorescence signals, and long-term effects, that is, reduced blastocyst formation rate. In vitrified oocytes, VB increased ROS levels. Prooxidant VB effects in ovine prepubertal oocytes could be related to higher VB accumulation, which was found as almost one thousand times higher than that reported in other cell systems in previous studies. Also, long exposure times of oocytes to VB, throughout the duration of in vitro maturation culture, may have contributed to significant increase of oocyte oxidation. Further studies are needed to identify lower concentrations and/or shorter exposure times to figure out VB antioxidant effects in juvenile ARTs. |
format | Online Article Text |
id | pubmed-3955694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39556942014-04-09 Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes Dell'Aquila, M. E. Bogliolo, L. Russo, R. Martino, N. A. Filioli Uranio, M. Ariu, F. Amati, F. Sardanelli, A. M. Linsalata, V. Ferruzzi, M. G. Cardinali, A. Minervini, F. Biomed Res Int Research Article Verbascoside (VB) is a bioactive polyphenol from olive oil mill wastewater with known antioxidant activity. Oxidative stress is an emerging problem in assisted reproductive technology (ART). Juvenile ART is a promising topic because, in farm animals, it reduces the generation gap and, in human reproductive medicine, it helps to overcome premature ovarian failure. The aim of this study was to test the effects of VB on the developmental competence of ovine prepubertal oocytes and the bioenergetic/oxidative stress status of fresh and vitrified oocytes. In fresh oocytes, VB exerted prooxidant short-term effects, that is, catalase activity increase and uncoupled increases of mitochondria and reactive oxygen species (ROS) fluorescence signals, and long-term effects, that is, reduced blastocyst formation rate. In vitrified oocytes, VB increased ROS levels. Prooxidant VB effects in ovine prepubertal oocytes could be related to higher VB accumulation, which was found as almost one thousand times higher than that reported in other cell systems in previous studies. Also, long exposure times of oocytes to VB, throughout the duration of in vitro maturation culture, may have contributed to significant increase of oocyte oxidation. Further studies are needed to identify lower concentrations and/or shorter exposure times to figure out VB antioxidant effects in juvenile ARTs. Hindawi Publishing Corporation 2014 2014-02-25 /pmc/articles/PMC3955694/ /pubmed/24719893 http://dx.doi.org/10.1155/2014/878062 Text en Copyright © 2014 M. E. Dell'Aquila et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dell'Aquila, M. E. Bogliolo, L. Russo, R. Martino, N. A. Filioli Uranio, M. Ariu, F. Amati, F. Sardanelli, A. M. Linsalata, V. Ferruzzi, M. G. Cardinali, A. Minervini, F. Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes |
title | Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes |
title_full | Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes |
title_fullStr | Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes |
title_full_unstemmed | Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes |
title_short | Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes |
title_sort | prooxidant effects of verbascoside, a bioactive compound from olive oil mill wastewater, on in vitro developmental potential of ovine prepubertal oocytes and bioenergetic/oxidative stress parameters of fresh and vitrified oocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955694/ https://www.ncbi.nlm.nih.gov/pubmed/24719893 http://dx.doi.org/10.1155/2014/878062 |
work_keys_str_mv | AT dellaquilame prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT bogliolol prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT russor prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT martinona prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT filioliuraniom prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT ariuf prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT amatif prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT sardanelliam prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT linsalatav prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT ferruzzimg prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT cardinalia prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes AT minervinif prooxidanteffectsofverbascosideabioactivecompoundfromoliveoilmillwastewateroninvitrodevelopmentalpotentialofovineprepubertaloocytesandbioenergeticoxidativestressparametersoffreshandvitrifiedoocytes |