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Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant extracts presents potential for preventive strategies against dental caries
Caries is a global public health problem, whose control requires the introduction of low-cost treatments, such as strong prevention strategies, minimally invasive techniques and chemical prevention agents. Nature plays an important role as a source of new antibacterial substances that can be used in...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculdade de Odontologia de Bauru da Universidade de São
Paulo
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956399/ https://www.ncbi.nlm.nih.gov/pubmed/24676578 http://dx.doi.org/10.1590/1678-775720130366 |
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author | da SILVA, Juliana Paola Corrêa de CASTILHO, Adriana Lígia SARACENI, Cíntia Helena Couri DÍAZ, Ingrit Elida Collantes PACIÊNCIA, Mateus Luís Barradas SUFFREDINI, Ivana Barbosa |
author_facet | da SILVA, Juliana Paola Corrêa de CASTILHO, Adriana Lígia SARACENI, Cíntia Helena Couri DÍAZ, Ingrit Elida Collantes PACIÊNCIA, Mateus Luís Barradas SUFFREDINI, Ivana Barbosa |
author_sort | da SILVA, Juliana Paola Corrêa |
collection | PubMed |
description | Caries is a global public health problem, whose control requires the introduction of low-cost treatments, such as strong prevention strategies, minimally invasive techniques and chemical prevention agents. Nature plays an important role as a source of new antibacterial substances that can be used in the prevention of caries, and Brazil is the richest country in terms of biodiversity. OBJECTIVE: In this study, the disk diffusion method (DDM) was used to screen over 2,000 Brazilian Amazon plant extracts against Streptococcus mutans. MATERIAL AND METHODS: Seventeen active plant extracts were identified and fractionated. Extracts and their fractions, obtained by liquid-liquid partition, were tested in the DDM assay and in the microdilution broth assay (MBA) to determine their minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs). The extracts were also subjected to antioxidant analysis by thin layer chromatography. RESULTS: EB271, obtained from Casearia spruceana, showed significant activity against the bacterium in the DDM assay (20.67±0.52 mm), as did EB1129, obtained from Psychotria sp. (Rubiaceae) (15.04±2.29 mm). EB1493, obtained from Ipomoea alba, was the only extract to show strong activity against Streptococcus mutans (0.08 mg/mL<MIC<0.16 mg/mL; MBC=0.16 mg/mL) in the MBA. CONCLUSIONS: The active extracts, discovered in the Amazon rain forest, show potential as sources of new antibacterial agents for use as chemical coadjuvants in prevention strategies to treat caries. |
format | Online Article Text |
id | pubmed-3956399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Faculdade de Odontologia de Bauru da Universidade de São
Paulo |
record_format | MEDLINE/PubMed |
spelling | pubmed-39563992014-03-18 Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant extracts presents potential for preventive strategies against dental caries da SILVA, Juliana Paola Corrêa de CASTILHO, Adriana Lígia SARACENI, Cíntia Helena Couri DÍAZ, Ingrit Elida Collantes PACIÊNCIA, Mateus Luís Barradas SUFFREDINI, Ivana Barbosa J Appl Oral Sci Original Articles Caries is a global public health problem, whose control requires the introduction of low-cost treatments, such as strong prevention strategies, minimally invasive techniques and chemical prevention agents. Nature plays an important role as a source of new antibacterial substances that can be used in the prevention of caries, and Brazil is the richest country in terms of biodiversity. OBJECTIVE: In this study, the disk diffusion method (DDM) was used to screen over 2,000 Brazilian Amazon plant extracts against Streptococcus mutans. MATERIAL AND METHODS: Seventeen active plant extracts were identified and fractionated. Extracts and their fractions, obtained by liquid-liquid partition, were tested in the DDM assay and in the microdilution broth assay (MBA) to determine their minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs). The extracts were also subjected to antioxidant analysis by thin layer chromatography. RESULTS: EB271, obtained from Casearia spruceana, showed significant activity against the bacterium in the DDM assay (20.67±0.52 mm), as did EB1129, obtained from Psychotria sp. (Rubiaceae) (15.04±2.29 mm). EB1493, obtained from Ipomoea alba, was the only extract to show strong activity against Streptococcus mutans (0.08 mg/mL<MIC<0.16 mg/mL; MBC=0.16 mg/mL) in the MBA. CONCLUSIONS: The active extracts, discovered in the Amazon rain forest, show potential as sources of new antibacterial agents for use as chemical coadjuvants in prevention strategies to treat caries. Faculdade de Odontologia de Bauru da Universidade de São Paulo 2014 /pmc/articles/PMC3956399/ /pubmed/24676578 http://dx.doi.org/10.1590/1678-775720130366 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles da SILVA, Juliana Paola Corrêa de CASTILHO, Adriana Lígia SARACENI, Cíntia Helena Couri DÍAZ, Ingrit Elida Collantes PACIÊNCIA, Mateus Luís Barradas SUFFREDINI, Ivana Barbosa Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant extracts presents potential for preventive strategies against dental caries |
title | Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant
extracts presents potential for preventive strategies against dental
caries |
title_full | Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant
extracts presents potential for preventive strategies against dental
caries |
title_fullStr | Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant
extracts presents potential for preventive strategies against dental
caries |
title_full_unstemmed | Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant
extracts presents potential for preventive strategies against dental
caries |
title_short | Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant
extracts presents potential for preventive strategies against dental
caries |
title_sort | anti-streptococcal activity of brazilian amazon rain forest plant
extracts presents potential for preventive strategies against dental
caries |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956399/ https://www.ncbi.nlm.nih.gov/pubmed/24676578 http://dx.doi.org/10.1590/1678-775720130366 |
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