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Arterial Injury and Endothelial Repair: Rapid Recovery of Function after Mechanical Injury in Healthy Volunteers

Objective. Previous studies suggest a protracted course of recovery after mechanical endothelial injury; confounders may include degree of injury and concomitant endothelial dysfunction. We sought to define the time course of endothelial function recovery using flow-mediated dilation (FMD), after is...

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Autores principales: Tilling, Lindsey, Hunt, Joanne, Donald, Ann, Clapp, Brian, Chowienczyk, Phil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956421/
https://www.ncbi.nlm.nih.gov/pubmed/24719774
http://dx.doi.org/10.1155/2014/367537
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author Tilling, Lindsey
Hunt, Joanne
Donald, Ann
Clapp, Brian
Chowienczyk, Phil
author_facet Tilling, Lindsey
Hunt, Joanne
Donald, Ann
Clapp, Brian
Chowienczyk, Phil
author_sort Tilling, Lindsey
collection PubMed
description Objective. Previous studies suggest a protracted course of recovery after mechanical endothelial injury; confounders may include degree of injury and concomitant endothelial dysfunction. We sought to define the time course of endothelial function recovery using flow-mediated dilation (FMD), after ischaemia-reperfusion (IR) and mechanical injury in patients and healthy volunteers. The contribution of circulating CD133(+)/CD34(+)/VEGFR2(+) “endothelial progenitor” (EPC) or repair cells to endothelial repair was also examined. Methods. 28 healthy volunteers aged 18–35 years underwent transient forearm ischaemia induced by cuff inflation around the proximal biceps and radial artery mechanical injury induced by inserting a wire through a cannula. A more severe mechanical injury was induced using an arterial sheath and catheter inserted into the radial artery of 18 patients undergoing angiography. Results. IR and mechanical injury produced immediate impairment of FMD (from 6.5 ± 1.2% to 2.9 ± 2.2% and from 7.4 ± 2.3% to 1.5 ± 1.6% for IR and injury, resp., each P < 0.001) but recovered within 6 hours and 2 days, respectively. FMD took up to 4 months to recover in patients. Circulating EPC did not change significantly during the injury/recovery period in all subjects. Conclusions. Recovery of endothelial function after IR and mechanical injury is rapid and not associated with a change in circulating EPC.
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spelling pubmed-39564212014-04-09 Arterial Injury and Endothelial Repair: Rapid Recovery of Function after Mechanical Injury in Healthy Volunteers Tilling, Lindsey Hunt, Joanne Donald, Ann Clapp, Brian Chowienczyk, Phil Cardiol Res Pract Clinical Study Objective. Previous studies suggest a protracted course of recovery after mechanical endothelial injury; confounders may include degree of injury and concomitant endothelial dysfunction. We sought to define the time course of endothelial function recovery using flow-mediated dilation (FMD), after ischaemia-reperfusion (IR) and mechanical injury in patients and healthy volunteers. The contribution of circulating CD133(+)/CD34(+)/VEGFR2(+) “endothelial progenitor” (EPC) or repair cells to endothelial repair was also examined. Methods. 28 healthy volunteers aged 18–35 years underwent transient forearm ischaemia induced by cuff inflation around the proximal biceps and radial artery mechanical injury induced by inserting a wire through a cannula. A more severe mechanical injury was induced using an arterial sheath and catheter inserted into the radial artery of 18 patients undergoing angiography. Results. IR and mechanical injury produced immediate impairment of FMD (from 6.5 ± 1.2% to 2.9 ± 2.2% and from 7.4 ± 2.3% to 1.5 ± 1.6% for IR and injury, resp., each P < 0.001) but recovered within 6 hours and 2 days, respectively. FMD took up to 4 months to recover in patients. Circulating EPC did not change significantly during the injury/recovery period in all subjects. Conclusions. Recovery of endothelial function after IR and mechanical injury is rapid and not associated with a change in circulating EPC. Hindawi Publishing Corporation 2014 2014-01-28 /pmc/articles/PMC3956421/ /pubmed/24719774 http://dx.doi.org/10.1155/2014/367537 Text en Copyright © 2014 Lindsey Tilling et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
Tilling, Lindsey
Hunt, Joanne
Donald, Ann
Clapp, Brian
Chowienczyk, Phil
Arterial Injury and Endothelial Repair: Rapid Recovery of Function after Mechanical Injury in Healthy Volunteers
title Arterial Injury and Endothelial Repair: Rapid Recovery of Function after Mechanical Injury in Healthy Volunteers
title_full Arterial Injury and Endothelial Repair: Rapid Recovery of Function after Mechanical Injury in Healthy Volunteers
title_fullStr Arterial Injury and Endothelial Repair: Rapid Recovery of Function after Mechanical Injury in Healthy Volunteers
title_full_unstemmed Arterial Injury and Endothelial Repair: Rapid Recovery of Function after Mechanical Injury in Healthy Volunteers
title_short Arterial Injury and Endothelial Repair: Rapid Recovery of Function after Mechanical Injury in Healthy Volunteers
title_sort arterial injury and endothelial repair: rapid recovery of function after mechanical injury in healthy volunteers
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956421/
https://www.ncbi.nlm.nih.gov/pubmed/24719774
http://dx.doi.org/10.1155/2014/367537
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