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Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis

Synechocystis sp. PCC6803 belongs to cyanobacteria which carry out photosynthesis and has recently become of interest due to the evolutionary link between bacteria and plant species. Similar to other bacteria, the primary carbohydrate storage source of Synechocystis sp. PCC6803 is glycogen. While mo...

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Autores principales: Yoo, Sang-Ho, Lee, Byung-Hoo, Moon, Youyoun, Spalding, Martin H., Jane, Jay-lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956634/
https://www.ncbi.nlm.nih.gov/pubmed/24637565
http://dx.doi.org/10.1371/journal.pone.0091524
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author Yoo, Sang-Ho
Lee, Byung-Hoo
Moon, Youyoun
Spalding, Martin H.
Jane, Jay-lin
author_facet Yoo, Sang-Ho
Lee, Byung-Hoo
Moon, Youyoun
Spalding, Martin H.
Jane, Jay-lin
author_sort Yoo, Sang-Ho
collection PubMed
description Synechocystis sp. PCC6803 belongs to cyanobacteria which carry out photosynthesis and has recently become of interest due to the evolutionary link between bacteria and plant species. Similar to other bacteria, the primary carbohydrate storage source of Synechocystis sp. PCC6803 is glycogen. While most bacteria are not known to have any isoforms of glycogen synthase, analysis of the genomic DNA sequence of Synechocystis sp. PCC6803 predicts that this strain encodes two isoforms of glycogen synthase (GS) for synthesizing glycogen structure. To examine the functions of the putative GS genes, each gene (sll1393 or sll0945) was disrupted by double cross-over homologous recombination. Zymogram analysis of the two GS disruption mutants allowed the identification of a protein band corresponding to each GS isoform. Results showed that two GS isoforms (GSI and GSII) are present in Synechocystis sp. PCC6803, and both are involved in glycogen biosynthesis with different elongation properties: GSI is processive and GSII is distributive. Total GS activities in the mutant strains were not affected and were compensated by the remaining isoform. Analysis of the branch-structure of glycogen revealed that the sll1393(−) mutant (GSI(−)) produced glycogen containing more intermediate-length chains (DP 8–18) at the expense of shorter and longer chains compared with the wild-type strain. The sll0945(−) mutant (GSII(−)) produced glycogen similar to the wild-type, with only a slightly higher proportion of short chains (DP 4–11). The current study suggests that GS isoforms in Synechocystis sp. PCC6803 have different elongation specificities in the biosynthesis of glycogen, combined with ADP-glucose pyrophosphorylase and glycogen branching enzyme.
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spelling pubmed-39566342014-03-18 Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis Yoo, Sang-Ho Lee, Byung-Hoo Moon, Youyoun Spalding, Martin H. Jane, Jay-lin PLoS One Research Article Synechocystis sp. PCC6803 belongs to cyanobacteria which carry out photosynthesis and has recently become of interest due to the evolutionary link between bacteria and plant species. Similar to other bacteria, the primary carbohydrate storage source of Synechocystis sp. PCC6803 is glycogen. While most bacteria are not known to have any isoforms of glycogen synthase, analysis of the genomic DNA sequence of Synechocystis sp. PCC6803 predicts that this strain encodes two isoforms of glycogen synthase (GS) for synthesizing glycogen structure. To examine the functions of the putative GS genes, each gene (sll1393 or sll0945) was disrupted by double cross-over homologous recombination. Zymogram analysis of the two GS disruption mutants allowed the identification of a protein band corresponding to each GS isoform. Results showed that two GS isoforms (GSI and GSII) are present in Synechocystis sp. PCC6803, and both are involved in glycogen biosynthesis with different elongation properties: GSI is processive and GSII is distributive. Total GS activities in the mutant strains were not affected and were compensated by the remaining isoform. Analysis of the branch-structure of glycogen revealed that the sll1393(−) mutant (GSI(−)) produced glycogen containing more intermediate-length chains (DP 8–18) at the expense of shorter and longer chains compared with the wild-type strain. The sll0945(−) mutant (GSII(−)) produced glycogen similar to the wild-type, with only a slightly higher proportion of short chains (DP 4–11). The current study suggests that GS isoforms in Synechocystis sp. PCC6803 have different elongation specificities in the biosynthesis of glycogen, combined with ADP-glucose pyrophosphorylase and glycogen branching enzyme. Public Library of Science 2014-03-17 /pmc/articles/PMC3956634/ /pubmed/24637565 http://dx.doi.org/10.1371/journal.pone.0091524 Text en © 2014 Yoo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yoo, Sang-Ho
Lee, Byung-Hoo
Moon, Youyoun
Spalding, Martin H.
Jane, Jay-lin
Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis
title Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis
title_full Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis
title_fullStr Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis
title_full_unstemmed Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis
title_short Glycogen Synthase Isoforms in Synechocystis sp. PCC6803: Identification of Different Roles to Produce Glycogen by Targeted Mutagenesis
title_sort glycogen synthase isoforms in synechocystis sp. pcc6803: identification of different roles to produce glycogen by targeted mutagenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956634/
https://www.ncbi.nlm.nih.gov/pubmed/24637565
http://dx.doi.org/10.1371/journal.pone.0091524
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