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Gene Expression Profiling in Winged and Wingless Cotton Aphids, Aphis gossypii (Hemiptera: Aphididae)

While trade-offs between flight capability and reproduction is a common phenomenon in wing dimorphic insects, the molecular basis is largely unknown. In this study, we examined the transcriptomic differences between winged and wingless morphs of cotton aphids, Aphis gossypii, using a tag-based digit...

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Autores principales: Yang, Xiaowei, Liu, Xiaoxia, Xu, Xiangli, Li, Zhen, Li, Yisong, Song, Dongyan, Yu, Tian, Zhu, Fang, Zhang, Qingwen, Zhou, Xuguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3957081/
https://www.ncbi.nlm.nih.gov/pubmed/24644424
http://dx.doi.org/10.7150/ijbs.7629
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author Yang, Xiaowei
Liu, Xiaoxia
Xu, Xiangli
Li, Zhen
Li, Yisong
Song, Dongyan
Yu, Tian
Zhu, Fang
Zhang, Qingwen
Zhou, Xuguo
author_facet Yang, Xiaowei
Liu, Xiaoxia
Xu, Xiangli
Li, Zhen
Li, Yisong
Song, Dongyan
Yu, Tian
Zhu, Fang
Zhang, Qingwen
Zhou, Xuguo
author_sort Yang, Xiaowei
collection PubMed
description While trade-offs between flight capability and reproduction is a common phenomenon in wing dimorphic insects, the molecular basis is largely unknown. In this study, we examined the transcriptomic differences between winged and wingless morphs of cotton aphids, Aphis gossypii, using a tag-based digital gene expression (DGE) approach. Ultra high-throughput Illumina sequencing generated 5.30 and 5.39 million raw tags, respectively, from winged and wingless A. gossypii DGE libraries. We identified 1,663 differentially expressed transcripts, among which 58 were highly expressed in the winged A. gossypii, whereas 1,605 expressed significantly higher in the wingless morphs. Bioinformatics tools, including Gene Ontology, Cluster of Orthologous Groups, euKaryotic Orthologous Groups and Kyoto Encyclopedia of Genes and Genomes pathways, were used to functionally annotate these transcripts. In addition, 20 differentially expressed transcripts detected by DGE were validated by the quantitative real-time PCR. Comparative transcriptomic analysis of sedentary (wingless) and migratory (winged) A. gossyii not only advances our understanding of the trade-offs in wing dimorphic insects, but also provides a candidate molecular target for the genetic control of this agricultural insect pest.
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spelling pubmed-39570812014-03-18 Gene Expression Profiling in Winged and Wingless Cotton Aphids, Aphis gossypii (Hemiptera: Aphididae) Yang, Xiaowei Liu, Xiaoxia Xu, Xiangli Li, Zhen Li, Yisong Song, Dongyan Yu, Tian Zhu, Fang Zhang, Qingwen Zhou, Xuguo Int J Biol Sci Research Paper While trade-offs between flight capability and reproduction is a common phenomenon in wing dimorphic insects, the molecular basis is largely unknown. In this study, we examined the transcriptomic differences between winged and wingless morphs of cotton aphids, Aphis gossypii, using a tag-based digital gene expression (DGE) approach. Ultra high-throughput Illumina sequencing generated 5.30 and 5.39 million raw tags, respectively, from winged and wingless A. gossypii DGE libraries. We identified 1,663 differentially expressed transcripts, among which 58 were highly expressed in the winged A. gossypii, whereas 1,605 expressed significantly higher in the wingless morphs. Bioinformatics tools, including Gene Ontology, Cluster of Orthologous Groups, euKaryotic Orthologous Groups and Kyoto Encyclopedia of Genes and Genomes pathways, were used to functionally annotate these transcripts. In addition, 20 differentially expressed transcripts detected by DGE were validated by the quantitative real-time PCR. Comparative transcriptomic analysis of sedentary (wingless) and migratory (winged) A. gossyii not only advances our understanding of the trade-offs in wing dimorphic insects, but also provides a candidate molecular target for the genetic control of this agricultural insect pest. Ivyspring International Publisher 2014-02-19 /pmc/articles/PMC3957081/ /pubmed/24644424 http://dx.doi.org/10.7150/ijbs.7629 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Yang, Xiaowei
Liu, Xiaoxia
Xu, Xiangli
Li, Zhen
Li, Yisong
Song, Dongyan
Yu, Tian
Zhu, Fang
Zhang, Qingwen
Zhou, Xuguo
Gene Expression Profiling in Winged and Wingless Cotton Aphids, Aphis gossypii (Hemiptera: Aphididae)
title Gene Expression Profiling in Winged and Wingless Cotton Aphids, Aphis gossypii (Hemiptera: Aphididae)
title_full Gene Expression Profiling in Winged and Wingless Cotton Aphids, Aphis gossypii (Hemiptera: Aphididae)
title_fullStr Gene Expression Profiling in Winged and Wingless Cotton Aphids, Aphis gossypii (Hemiptera: Aphididae)
title_full_unstemmed Gene Expression Profiling in Winged and Wingless Cotton Aphids, Aphis gossypii (Hemiptera: Aphididae)
title_short Gene Expression Profiling in Winged and Wingless Cotton Aphids, Aphis gossypii (Hemiptera: Aphididae)
title_sort gene expression profiling in winged and wingless cotton aphids, aphis gossypii (hemiptera: aphididae)
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3957081/
https://www.ncbi.nlm.nih.gov/pubmed/24644424
http://dx.doi.org/10.7150/ijbs.7629
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