FlyGEM, a full transcriptome array platform for the Drosophila community

We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been ext...

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Detalles Bibliográficos
Autores principales: Johnston, Rick, Wang, Bruce, Nuttall, Rachel, Doctolero, Michael, Edwards, Pamela, Lü, Jining, Vainer, Marina, Yue, Huibin, Wang, Xinhao, Minor, James, Chan, Cathy, Lash, Alex, Goralski, Thomas, Parisi, Michael, Oliver, Brian, Eastman, Scott
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC395769/
https://www.ncbi.nlm.nih.gov/pubmed/15003122
Descripción
Sumario:We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.

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