FlyGEM, a full transcriptome array platform for the Drosophila community

We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been ext...

Descripción completa

Detalles Bibliográficos
Autores principales: Johnston, Rick, Wang, Bruce, Nuttall, Rachel, Doctolero, Michael, Edwards, Pamela, Lü, Jining, Vainer, Marina, Yue, Huibin, Wang, Xinhao, Minor, James, Chan, Cathy, Lash, Alex, Goralski, Thomas, Parisi, Michael, Oliver, Brian, Eastman, Scott
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC395769/
https://www.ncbi.nlm.nih.gov/pubmed/15003122
_version_ 1782121330982780928
author Johnston, Rick
Wang, Bruce
Nuttall, Rachel
Doctolero, Michael
Edwards, Pamela
Lü, Jining
Vainer, Marina
Yue, Huibin
Wang, Xinhao
Minor, James
Chan, Cathy
Lash, Alex
Goralski, Thomas
Parisi, Michael
Oliver, Brian
Eastman, Scott
author_facet Johnston, Rick
Wang, Bruce
Nuttall, Rachel
Doctolero, Michael
Edwards, Pamela
Lü, Jining
Vainer, Marina
Yue, Huibin
Wang, Xinhao
Minor, James
Chan, Cathy
Lash, Alex
Goralski, Thomas
Parisi, Michael
Oliver, Brian
Eastman, Scott
author_sort Johnston, Rick
collection PubMed
description We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.
format Text
id pubmed-395769
institution National Center for Biotechnology Information
language English
publishDate 2004
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-3957692004-04-24 FlyGEM, a full transcriptome array platform for the Drosophila community Johnston, Rick Wang, Bruce Nuttall, Rachel Doctolero, Michael Edwards, Pamela Lü, Jining Vainer, Marina Yue, Huibin Wang, Xinhao Minor, James Chan, Cathy Lash, Alex Goralski, Thomas Parisi, Michael Oliver, Brian Eastman, Scott Genome Biol Method We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee. BioMed Central 2004 2004-02-26 /pmc/articles/PMC395769/ /pubmed/15003122 Text en Copyright © 2004 Johnston et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Method
Johnston, Rick
Wang, Bruce
Nuttall, Rachel
Doctolero, Michael
Edwards, Pamela
Lü, Jining
Vainer, Marina
Yue, Huibin
Wang, Xinhao
Minor, James
Chan, Cathy
Lash, Alex
Goralski, Thomas
Parisi, Michael
Oliver, Brian
Eastman, Scott
FlyGEM, a full transcriptome array platform for the Drosophila community
title FlyGEM, a full transcriptome array platform for the Drosophila community
title_full FlyGEM, a full transcriptome array platform for the Drosophila community
title_fullStr FlyGEM, a full transcriptome array platform for the Drosophila community
title_full_unstemmed FlyGEM, a full transcriptome array platform for the Drosophila community
title_short FlyGEM, a full transcriptome array platform for the Drosophila community
title_sort flygem, a full transcriptome array platform for the drosophila community
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC395769/
https://www.ncbi.nlm.nih.gov/pubmed/15003122
work_keys_str_mv AT johnstonrick flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT wangbruce flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT nuttallrachel flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT doctoleromichael flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT edwardspamela flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT lujining flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT vainermarina flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT yuehuibin flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT wangxinhao flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT minorjames flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT chancathy flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT lashalex flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT goralskithomas flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT parisimichael flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT oliverbrian flygemafulltranscriptomearrayplatformforthedrosophilacommunity
AT eastmanscott flygemafulltranscriptomearrayplatformforthedrosophilacommunity

Ejemplares similares