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An oxidant and organic solvent tolerant alkaline lipase by P. aeruginosa mutant: Downstream processing and biochemical characterization
An extracellular alkaline lipase from Pseudomonas aeruginosa mutant has been purified to homogeneity using acetone precipitation followed by anion exchange and gel filtration chromatography and resulted in 27-fold purification with 19.6% final recovery. SDS-PAGE study suggested that the purified lip...
Autores principales: | Bisht, Deepali, Yadav, Santosh Kumar, Darmwal, Nandan Singh |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Brazilian Society of Microbiology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958203/ https://www.ncbi.nlm.nih.gov/pubmed/24688527 |
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