Cargando…

Low False-Positives in an mLumin-Based Bimolecular Fluorescence Complementation System with a Bicistronic Expression Vector

The simplicity and sensitivity of the bimolecular fluorescence complementation (BiFC) assay make it a powerful tool to investigate protein-protein interactions (PPIs) in living cells. However, non-specific association of the fluorescent protein fragments in a BiFC system can complicate evaluation of...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Shun, Li, Xiangyong, Yang, Jie, Zhang, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958255/
https://www.ncbi.nlm.nih.gov/pubmed/24556667
http://dx.doi.org/10.3390/s140203284