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Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy
Campylobacter has developed resistance to several antimicrobial agents over the years, including macrolides, quinolones and fluoroquinolones, becoming a significant public health hazard. A total of 145 strains derived from raw milk, chicken faeces, chicken carcasses, cattle faeces and human faeces c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958300/ https://www.ncbi.nlm.nih.gov/pubmed/24556669 http://dx.doi.org/10.3390/s140203308 |
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author | Di Giannatale, Elisabetta Di Serafino, Gabriella Zilli, Katiuscia Alessiani, Alessandra Sacchini, Lorena Garofolo, Giuliano Aprea, Giuseppe Marotta, Francesca |
author_facet | Di Giannatale, Elisabetta Di Serafino, Gabriella Zilli, Katiuscia Alessiani, Alessandra Sacchini, Lorena Garofolo, Giuliano Aprea, Giuseppe Marotta, Francesca |
author_sort | Di Giannatale, Elisabetta |
collection | PubMed |
description | Campylobacter has developed resistance to several antimicrobial agents over the years, including macrolides, quinolones and fluoroquinolones, becoming a significant public health hazard. A total of 145 strains derived from raw milk, chicken faeces, chicken carcasses, cattle faeces and human faeces collected from various Italian regions, were screened for antimicrobial susceptibility, molecular characterization (SmaI pulsed-field gel electrophoresis) and detection of virulence genes (sequencing and DNA microarray analysis). The prevalence of C. jejuni and C. coli was 62.75% and 37.24% respectively. Antimicrobial susceptibility revealed a high level of resistance for ciprofloxacin (62.76%), tetracycline (55.86%) and nalidixic acid (55.17%). Genotyping of Campylobacter isolates using PFGE revealed a total of 86 unique SmaI patterns. Virulence gene profiles were determined using a new microbial diagnostic microarray composed of 70-mer oligonucleotide probes targeting genes implicated in Campylobacter pathogenicity. Correspondence between PFGE and microarray clusters was observed. Comparisons of PFGE and virulence profiles reflected the high genetic diversity of the strains examined, leading us to speculate different degrees of pathogenicity inside Campylobacter populations. |
format | Online Article Text |
id | pubmed-3958300 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-39583002014-03-20 Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy Di Giannatale, Elisabetta Di Serafino, Gabriella Zilli, Katiuscia Alessiani, Alessandra Sacchini, Lorena Garofolo, Giuliano Aprea, Giuseppe Marotta, Francesca Sensors (Basel) Article Campylobacter has developed resistance to several antimicrobial agents over the years, including macrolides, quinolones and fluoroquinolones, becoming a significant public health hazard. A total of 145 strains derived from raw milk, chicken faeces, chicken carcasses, cattle faeces and human faeces collected from various Italian regions, were screened for antimicrobial susceptibility, molecular characterization (SmaI pulsed-field gel electrophoresis) and detection of virulence genes (sequencing and DNA microarray analysis). The prevalence of C. jejuni and C. coli was 62.75% and 37.24% respectively. Antimicrobial susceptibility revealed a high level of resistance for ciprofloxacin (62.76%), tetracycline (55.86%) and nalidixic acid (55.17%). Genotyping of Campylobacter isolates using PFGE revealed a total of 86 unique SmaI patterns. Virulence gene profiles were determined using a new microbial diagnostic microarray composed of 70-mer oligonucleotide probes targeting genes implicated in Campylobacter pathogenicity. Correspondence between PFGE and microarray clusters was observed. Comparisons of PFGE and virulence profiles reflected the high genetic diversity of the strains examined, leading us to speculate different degrees of pathogenicity inside Campylobacter populations. Molecular Diversity Preservation International (MDPI) 2014-02-19 /pmc/articles/PMC3958300/ /pubmed/24556669 http://dx.doi.org/10.3390/s140203308 Text en © 2014 by the authors; licensee MDPI; Basel; Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Di Giannatale, Elisabetta Di Serafino, Gabriella Zilli, Katiuscia Alessiani, Alessandra Sacchini, Lorena Garofolo, Giuliano Aprea, Giuseppe Marotta, Francesca Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy |
title | Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy |
title_full | Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy |
title_fullStr | Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy |
title_full_unstemmed | Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy |
title_short | Characterization of Antimicrobial Resistance Patterns and Detection of Virulence Genes in Campylobacter Isolates in Italy |
title_sort | characterization of antimicrobial resistance patterns and detection of virulence genes in campylobacter isolates in italy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958300/ https://www.ncbi.nlm.nih.gov/pubmed/24556669 http://dx.doi.org/10.3390/s140203308 |
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