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Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 μM) in human lymp...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958316/ https://www.ncbi.nlm.nih.gov/pubmed/24688297 |
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author | Alvarez-Moya, Carlos Silva, Mónica Reynoso Ramírez, Carlos Valdez Gallardo, David Gómez Sánchez, Rafael León Aguirre, Alejandro Canales Velasco, Alfredo Feria |
author_facet | Alvarez-Moya, Carlos Silva, Mónica Reynoso Ramírez, Carlos Valdez Gallardo, David Gómez Sánchez, Rafael León Aguirre, Alejandro Canales Velasco, Alfredo Feria |
author_sort | Alvarez-Moya, Carlos |
collection | PubMed |
description | There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 μM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p < 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared to in vitro, possibly because of efficient metabolization of the herbicide. In O. niloticus erythrocytes, significant (p < 0.001) genotoxicity was observed at ≥ 7 μM, whereas in vitro, glyphosphate was genotoxic in human lymphocytes and Tradescantia hairs at ≥ 0.7 μM. These results indicate that glyphosate is genotoxic in the cells and organisms studied at concentrations of 0.7–7 μM. |
format | Online Article Text |
id | pubmed-3958316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-39583162014-03-31 Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms Alvarez-Moya, Carlos Silva, Mónica Reynoso Ramírez, Carlos Valdez Gallardo, David Gómez Sánchez, Rafael León Aguirre, Alejandro Canales Velasco, Alfredo Feria Genet Mol Biol Mutagenesis There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 μM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p < 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared to in vitro, possibly because of efficient metabolization of the herbicide. In O. niloticus erythrocytes, significant (p < 0.001) genotoxicity was observed at ≥ 7 μM, whereas in vitro, glyphosphate was genotoxic in human lymphocytes and Tradescantia hairs at ≥ 0.7 μM. These results indicate that glyphosate is genotoxic in the cells and organisms studied at concentrations of 0.7–7 μM. Sociedade Brasileira de Genética 2014-03 2013-02-28 /pmc/articles/PMC3958316/ /pubmed/24688297 Text en Copyright © 2014, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Mutagenesis Alvarez-Moya, Carlos Silva, Mónica Reynoso Ramírez, Carlos Valdez Gallardo, David Gómez Sánchez, Rafael León Aguirre, Alejandro Canales Velasco, Alfredo Feria Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms |
title | Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms |
title_full | Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms |
title_fullStr | Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms |
title_full_unstemmed | Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms |
title_short | Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms |
title_sort | comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms |
topic | Mutagenesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958316/ https://www.ncbi.nlm.nih.gov/pubmed/24688297 |
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