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Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms

There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 μM) in human lymp...

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Autores principales: Alvarez-Moya, Carlos, Silva, Mónica Reynoso, Ramírez, Carlos Valdez, Gallardo, David Gómez, Sánchez, Rafael León, Aguirre, Alejandro Canales, Velasco, Alfredo Feria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958316/
https://www.ncbi.nlm.nih.gov/pubmed/24688297
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author Alvarez-Moya, Carlos
Silva, Mónica Reynoso
Ramírez, Carlos Valdez
Gallardo, David Gómez
Sánchez, Rafael León
Aguirre, Alejandro Canales
Velasco, Alfredo Feria
author_facet Alvarez-Moya, Carlos
Silva, Mónica Reynoso
Ramírez, Carlos Valdez
Gallardo, David Gómez
Sánchez, Rafael León
Aguirre, Alejandro Canales
Velasco, Alfredo Feria
author_sort Alvarez-Moya, Carlos
collection PubMed
description There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 μM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p < 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared to in vitro, possibly because of efficient metabolization of the herbicide. In O. niloticus erythrocytes, significant (p < 0.001) genotoxicity was observed at ≥ 7 μM, whereas in vitro, glyphosphate was genotoxic in human lymphocytes and Tradescantia hairs at ≥ 0.7 μM. These results indicate that glyphosate is genotoxic in the cells and organisms studied at concentrations of 0.7–7 μM.
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spelling pubmed-39583162014-03-31 Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms Alvarez-Moya, Carlos Silva, Mónica Reynoso Ramírez, Carlos Valdez Gallardo, David Gómez Sánchez, Rafael León Aguirre, Alejandro Canales Velasco, Alfredo Feria Genet Mol Biol Mutagenesis There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 μM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p < 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared to in vitro, possibly because of efficient metabolization of the herbicide. In O. niloticus erythrocytes, significant (p < 0.001) genotoxicity was observed at ≥ 7 μM, whereas in vitro, glyphosphate was genotoxic in human lymphocytes and Tradescantia hairs at ≥ 0.7 μM. These results indicate that glyphosate is genotoxic in the cells and organisms studied at concentrations of 0.7–7 μM. Sociedade Brasileira de Genética 2014-03 2013-02-28 /pmc/articles/PMC3958316/ /pubmed/24688297 Text en Copyright © 2014, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mutagenesis
Alvarez-Moya, Carlos
Silva, Mónica Reynoso
Ramírez, Carlos Valdez
Gallardo, David Gómez
Sánchez, Rafael León
Aguirre, Alejandro Canales
Velasco, Alfredo Feria
Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
title Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
title_full Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
title_fullStr Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
title_full_unstemmed Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
title_short Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
title_sort comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
topic Mutagenesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958316/
https://www.ncbi.nlm.nih.gov/pubmed/24688297
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