Cargando…
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites fo...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958326/ https://www.ncbi.nlm.nih.gov/pubmed/24688291 |
_version_ | 1782307852050759680 |
---|---|
author | da Silva, Elizabete Cristina McManus, Concepta Margaret de Paiva Guimarães, Maria Pia Souza Lima Mattos Gouveia, Aurora M.G. Facó, Olivardo Pimentel, Daniel M. Caetano, Alexandre Rodrigues Paiva, Samuel Rezende |
author_facet | da Silva, Elizabete Cristina McManus, Concepta Margaret de Paiva Guimarães, Maria Pia Souza Lima Mattos Gouveia, Aurora M.G. Facó, Olivardo Pimentel, Daniel M. Caetano, Alexandre Rodrigues Paiva, Samuel Rezende |
author_sort | da Silva, Elizabete Cristina |
collection | PubMed |
description | Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided. |
format | Online Article Text |
id | pubmed-3958326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-39583262014-03-31 Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil da Silva, Elizabete Cristina McManus, Concepta Margaret de Paiva Guimarães, Maria Pia Souza Lima Mattos Gouveia, Aurora M.G. Facó, Olivardo Pimentel, Daniel M. Caetano, Alexandre Rodrigues Paiva, Samuel Rezende Genet Mol Biol Animal Genetics Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided. Sociedade Brasileira de Genética 2014-03 2013-02-28 /pmc/articles/PMC3958326/ /pubmed/24688291 Text en Copyright © 2014, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Animal Genetics da Silva, Elizabete Cristina McManus, Concepta Margaret de Paiva Guimarães, Maria Pia Souza Lima Mattos Gouveia, Aurora M.G. Facó, Olivardo Pimentel, Daniel M. Caetano, Alexandre Rodrigues Paiva, Samuel Rezende Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title | Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_full | Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_fullStr | Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_full_unstemmed | Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_short | Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_sort | validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in brazil |
topic | Animal Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958326/ https://www.ncbi.nlm.nih.gov/pubmed/24688291 |
work_keys_str_mv | AT dasilvaelizabetecristina validationofamicrosatellitepanelforparentagetestingoflocallyadaptedandcommercialgoatsinbrazil AT mcmanusconceptamargaret validationofamicrosatellitepanelforparentagetestingoflocallyadaptedandcommercialgoatsinbrazil AT depaivaguimaraesmariapiasouzalimamattos validationofamicrosatellitepanelforparentagetestingoflocallyadaptedandcommercialgoatsinbrazil AT gouveiaauroramg validationofamicrosatellitepanelforparentagetestingoflocallyadaptedandcommercialgoatsinbrazil AT facoolivardo validationofamicrosatellitepanelforparentagetestingoflocallyadaptedandcommercialgoatsinbrazil AT pimenteldanielm validationofamicrosatellitepanelforparentagetestingoflocallyadaptedandcommercialgoatsinbrazil AT caetanoalexandrerodrigues validationofamicrosatellitepanelforparentagetestingoflocallyadaptedandcommercialgoatsinbrazil AT paivasamuelrezende validationofamicrosatellitepanelforparentagetestingoflocallyadaptedandcommercialgoatsinbrazil |