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Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L.
Jasmonates (JAs) are important signaling molecules in plants and play crucial roles in stress responses, secondary metabolites' regulation, plant growth and development. In this study, the promoter of AaAOC, which was the key gene of jasmonate biosynthetic pathway, had been cloned. GUS staining...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958394/ https://www.ncbi.nlm.nih.gov/pubmed/24642483 http://dx.doi.org/10.1371/journal.pone.0091741 |
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author | Lu, Xu Zhang, Fangyuan Shen, Qian Jiang, Weimin Pan, Qifang Lv, Zongyou Yan, Tingxiang Fu, Xueqing Wang, Yuliang Qian, Hongmei Tang, Kexuan |
author_facet | Lu, Xu Zhang, Fangyuan Shen, Qian Jiang, Weimin Pan, Qifang Lv, Zongyou Yan, Tingxiang Fu, Xueqing Wang, Yuliang Qian, Hongmei Tang, Kexuan |
author_sort | Lu, Xu |
collection | PubMed |
description | Jasmonates (JAs) are important signaling molecules in plants and play crucial roles in stress responses, secondary metabolites' regulation, plant growth and development. In this study, the promoter of AaAOC, which was the key gene of jasmonate biosynthetic pathway, had been cloned. GUS staining showed that AaAOC was expressed ubiquitiously in A. annua. AaAOC gene was overexpressed under control of 35S promoter. RT-Q-PCR showed that the expression levels of AaAOC were increased from 1.6- to 5.2-fold in AaAOC-overexpression transgenic A. annua. The results of GC-MS showed that the content of endogenous jasmonic acid (JA) was 2- to 4.7-fold of the control level in AaAOC-overexpression plants. HPLC showed that the contents of artemisinin, dihydroartemisinic acid and artemisinic acid were increased significantly in AaAOC-overexpression plants. RT-Q-PCR showed that the expression levels of FPS (farnesyl diphosphate synthase), CYP71AV1 (cytochrome P450 dependent hydroxylase) and DBR2 (double bond reductase 2) were increased significantly in AaAOC-overexpression plants. All data demonstrated that increased endogenous JA could significantly promote the biosynthesis of artemisinin in AaAOC-overexpression transgenic A.annua. |
format | Online Article Text |
id | pubmed-3958394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39583942014-03-24 Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L. Lu, Xu Zhang, Fangyuan Shen, Qian Jiang, Weimin Pan, Qifang Lv, Zongyou Yan, Tingxiang Fu, Xueqing Wang, Yuliang Qian, Hongmei Tang, Kexuan PLoS One Research Article Jasmonates (JAs) are important signaling molecules in plants and play crucial roles in stress responses, secondary metabolites' regulation, plant growth and development. In this study, the promoter of AaAOC, which was the key gene of jasmonate biosynthetic pathway, had been cloned. GUS staining showed that AaAOC was expressed ubiquitiously in A. annua. AaAOC gene was overexpressed under control of 35S promoter. RT-Q-PCR showed that the expression levels of AaAOC were increased from 1.6- to 5.2-fold in AaAOC-overexpression transgenic A. annua. The results of GC-MS showed that the content of endogenous jasmonic acid (JA) was 2- to 4.7-fold of the control level in AaAOC-overexpression plants. HPLC showed that the contents of artemisinin, dihydroartemisinic acid and artemisinic acid were increased significantly in AaAOC-overexpression plants. RT-Q-PCR showed that the expression levels of FPS (farnesyl diphosphate synthase), CYP71AV1 (cytochrome P450 dependent hydroxylase) and DBR2 (double bond reductase 2) were increased significantly in AaAOC-overexpression plants. All data demonstrated that increased endogenous JA could significantly promote the biosynthesis of artemisinin in AaAOC-overexpression transgenic A.annua. Public Library of Science 2014-03-18 /pmc/articles/PMC3958394/ /pubmed/24642483 http://dx.doi.org/10.1371/journal.pone.0091741 Text en © 2014 Lu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lu, Xu Zhang, Fangyuan Shen, Qian Jiang, Weimin Pan, Qifang Lv, Zongyou Yan, Tingxiang Fu, Xueqing Wang, Yuliang Qian, Hongmei Tang, Kexuan Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L. |
title | Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L. |
title_full | Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L. |
title_fullStr | Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L. |
title_full_unstemmed | Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L. |
title_short | Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L. |
title_sort | overexpression of allene oxide cyclase improves the biosynthesis of artemisinin in artemisia annua l. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958394/ https://www.ncbi.nlm.nih.gov/pubmed/24642483 http://dx.doi.org/10.1371/journal.pone.0091741 |
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