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Subcellular Localization of ENS-1/ERNI in Chick Embryonic Stem Cells

The protein of retroviral origin ENS-1/ERNI plays a major role during neural plate development in chick embryos by controlling the activity of the epigenetic regulator HP1γ, but its function in the earlier developmental stages is still unknown. ENS-1/ERNI promoter activity is down-regulated upon dif...

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Autores principales: Blanc, Sophie, Ruggiero, Florence, Birot, Anne-Marie, Acloque, Hervé, Décimo, Didier, Lerat, Emmanuelle, Ohlmann, Théophile, Samarut, Jacques, Mey, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958431/
https://www.ncbi.nlm.nih.gov/pubmed/24643087
http://dx.doi.org/10.1371/journal.pone.0092039
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author Blanc, Sophie
Ruggiero, Florence
Birot, Anne-Marie
Acloque, Hervé
Décimo, Didier
Lerat, Emmanuelle
Ohlmann, Théophile
Samarut, Jacques
Mey, Anne
author_facet Blanc, Sophie
Ruggiero, Florence
Birot, Anne-Marie
Acloque, Hervé
Décimo, Didier
Lerat, Emmanuelle
Ohlmann, Théophile
Samarut, Jacques
Mey, Anne
author_sort Blanc, Sophie
collection PubMed
description The protein of retroviral origin ENS-1/ERNI plays a major role during neural plate development in chick embryos by controlling the activity of the epigenetic regulator HP1γ, but its function in the earlier developmental stages is still unknown. ENS-1/ERNI promoter activity is down-regulated upon differentiation but the resulting protein expression has never been examined. In this study, we present the results obtained with custom-made antibodies to gain further insights into ENS-1 protein expression in Chicken embryonic stem cells (CES) and during their differentiation. First, we show that ENS-1 controls the activity of HP1γ in CES and we examined the context of its interaction with HP1γ. By combining immunofluorescence and western blot analysis we show that ENS-1 is localized in the cytoplasm and in the nucleus, in agreement with its role on gene's promoter activity. During differentiation, ENS-1 decreases in the cytoplasm but not in the nucleus. More precisely, three distinct forms of the ENS-1 protein co-exist in the nucleus and are differently regulated during differentiation, revealing a new level of control of the protein ENS-1. In silico analysis of the Ens-1 gene copies and the sequence of their corresponding proteins indicate that this pattern is compatible with at least three potential regulation mechanisms, each accounting only partially. The results obtained with the anti-ENS-1 antibodies presented here reveal that the regulation of ENS-1 expression in CES is more complex than expected, providing new tracks to explore the integration of ENS-1 in CES cells regulatory networks.
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spelling pubmed-39584312014-03-24 Subcellular Localization of ENS-1/ERNI in Chick Embryonic Stem Cells Blanc, Sophie Ruggiero, Florence Birot, Anne-Marie Acloque, Hervé Décimo, Didier Lerat, Emmanuelle Ohlmann, Théophile Samarut, Jacques Mey, Anne PLoS One Research Article The protein of retroviral origin ENS-1/ERNI plays a major role during neural plate development in chick embryos by controlling the activity of the epigenetic regulator HP1γ, but its function in the earlier developmental stages is still unknown. ENS-1/ERNI promoter activity is down-regulated upon differentiation but the resulting protein expression has never been examined. In this study, we present the results obtained with custom-made antibodies to gain further insights into ENS-1 protein expression in Chicken embryonic stem cells (CES) and during their differentiation. First, we show that ENS-1 controls the activity of HP1γ in CES and we examined the context of its interaction with HP1γ. By combining immunofluorescence and western blot analysis we show that ENS-1 is localized in the cytoplasm and in the nucleus, in agreement with its role on gene's promoter activity. During differentiation, ENS-1 decreases in the cytoplasm but not in the nucleus. More precisely, three distinct forms of the ENS-1 protein co-exist in the nucleus and are differently regulated during differentiation, revealing a new level of control of the protein ENS-1. In silico analysis of the Ens-1 gene copies and the sequence of their corresponding proteins indicate that this pattern is compatible with at least three potential regulation mechanisms, each accounting only partially. The results obtained with the anti-ENS-1 antibodies presented here reveal that the regulation of ENS-1 expression in CES is more complex than expected, providing new tracks to explore the integration of ENS-1 in CES cells regulatory networks. Public Library of Science 2014-03-18 /pmc/articles/PMC3958431/ /pubmed/24643087 http://dx.doi.org/10.1371/journal.pone.0092039 Text en © 2014 Blanc et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Blanc, Sophie
Ruggiero, Florence
Birot, Anne-Marie
Acloque, Hervé
Décimo, Didier
Lerat, Emmanuelle
Ohlmann, Théophile
Samarut, Jacques
Mey, Anne
Subcellular Localization of ENS-1/ERNI in Chick Embryonic Stem Cells
title Subcellular Localization of ENS-1/ERNI in Chick Embryonic Stem Cells
title_full Subcellular Localization of ENS-1/ERNI in Chick Embryonic Stem Cells
title_fullStr Subcellular Localization of ENS-1/ERNI in Chick Embryonic Stem Cells
title_full_unstemmed Subcellular Localization of ENS-1/ERNI in Chick Embryonic Stem Cells
title_short Subcellular Localization of ENS-1/ERNI in Chick Embryonic Stem Cells
title_sort subcellular localization of ens-1/erni in chick embryonic stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958431/
https://www.ncbi.nlm.nih.gov/pubmed/24643087
http://dx.doi.org/10.1371/journal.pone.0092039
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