Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate

The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged...

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Detalles Bibliográficos
Autores principales: Celestino, Jessyca dos Reis, Duarte, Ana Caroline, Silva, Cláudia Maria de Melo, Sena, Hellen Holanda, Ferreira, Maria do Perpétuo Socorro Borges Carriço, Mallmann, Neila Hiraishi, Lima, Natacha Pinheiro Costa, Tavares, Chanderlei de Castro, de Souza, Rodrigo Otávio Silva, Souza, Érica Simplício, Souza, João Vicente Braga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958758/
https://www.ncbi.nlm.nih.gov/pubmed/24724017
http://dx.doi.org/10.1155/2014/725651
Descripción
Sumario:The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening assay of starch hydrolysis in petri dishes (ASHPD) and in the assay in submerged fermentation (ASbF). The strain Aspergillus 6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of Aspergillus 6V4 amylases (385 IU/g) in solid state fermentation (SSF).

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