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Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate

The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged...

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Autores principales: Celestino, Jessyca dos Reis, Duarte, Ana Caroline, Silva, Cláudia Maria de Melo, Sena, Hellen Holanda, Ferreira, Maria do Perpétuo Socorro Borges Carriço, Mallmann, Neila Hiraishi, Lima, Natacha Pinheiro Costa, Tavares, Chanderlei de Castro, de Souza, Rodrigo Otávio Silva, Souza, Érica Simplício, Souza, João Vicente Braga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958758/
https://www.ncbi.nlm.nih.gov/pubmed/24724017
http://dx.doi.org/10.1155/2014/725651
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author Celestino, Jessyca dos Reis
Duarte, Ana Caroline
Silva, Cláudia Maria de Melo
Sena, Hellen Holanda
Ferreira, Maria do Perpétuo Socorro Borges Carriço
Mallmann, Neila Hiraishi
Lima, Natacha Pinheiro Costa
Tavares, Chanderlei de Castro
de Souza, Rodrigo Otávio Silva
Souza, Érica Simplício
Souza, João Vicente Braga
author_facet Celestino, Jessyca dos Reis
Duarte, Ana Caroline
Silva, Cláudia Maria de Melo
Sena, Hellen Holanda
Ferreira, Maria do Perpétuo Socorro Borges Carriço
Mallmann, Neila Hiraishi
Lima, Natacha Pinheiro Costa
Tavares, Chanderlei de Castro
de Souza, Rodrigo Otávio Silva
Souza, Érica Simplício
Souza, João Vicente Braga
author_sort Celestino, Jessyca dos Reis
collection PubMed
description The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening assay of starch hydrolysis in petri dishes (ASHPD) and in the assay in submerged fermentation (ASbF). The strain Aspergillus 6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of Aspergillus 6V4 amylases (385 IU/g) in solid state fermentation (SSF).
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spelling pubmed-39587582014-04-10 Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate Celestino, Jessyca dos Reis Duarte, Ana Caroline Silva, Cláudia Maria de Melo Sena, Hellen Holanda Ferreira, Maria do Perpétuo Socorro Borges Carriço Mallmann, Neila Hiraishi Lima, Natacha Pinheiro Costa Tavares, Chanderlei de Castro de Souza, Rodrigo Otávio Silva Souza, Érica Simplício Souza, João Vicente Braga Enzyme Res Research Article The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening assay of starch hydrolysis in petri dishes (ASHPD) and in the assay in submerged fermentation (ASbF). The strain Aspergillus 6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of Aspergillus 6V4 amylases (385 IU/g) in solid state fermentation (SSF). Hindawi Publishing Corporation 2014 2014-03-02 /pmc/articles/PMC3958758/ /pubmed/24724017 http://dx.doi.org/10.1155/2014/725651 Text en Copyright © 2014 Jessyca dos Reis Celestino et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Celestino, Jessyca dos Reis
Duarte, Ana Caroline
Silva, Cláudia Maria de Melo
Sena, Hellen Holanda
Ferreira, Maria do Perpétuo Socorro Borges Carriço
Mallmann, Neila Hiraishi
Lima, Natacha Pinheiro Costa
Tavares, Chanderlei de Castro
de Souza, Rodrigo Otávio Silva
Souza, Érica Simplício
Souza, João Vicente Braga
Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate
title Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate
title_full Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate
title_fullStr Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate
title_full_unstemmed Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate
title_short Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate
title_sort aspergillus 6v4, a strain isolated from manipueira, produces high amylases levels by using wheat bran as a substrate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958758/
https://www.ncbi.nlm.nih.gov/pubmed/24724017
http://dx.doi.org/10.1155/2014/725651
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