Cargando…
Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate
The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958758/ https://www.ncbi.nlm.nih.gov/pubmed/24724017 http://dx.doi.org/10.1155/2014/725651 |
_version_ | 1782307935022481408 |
---|---|
author | Celestino, Jessyca dos Reis Duarte, Ana Caroline Silva, Cláudia Maria de Melo Sena, Hellen Holanda Ferreira, Maria do Perpétuo Socorro Borges Carriço Mallmann, Neila Hiraishi Lima, Natacha Pinheiro Costa Tavares, Chanderlei de Castro de Souza, Rodrigo Otávio Silva Souza, Érica Simplício Souza, João Vicente Braga |
author_facet | Celestino, Jessyca dos Reis Duarte, Ana Caroline Silva, Cláudia Maria de Melo Sena, Hellen Holanda Ferreira, Maria do Perpétuo Socorro Borges Carriço Mallmann, Neila Hiraishi Lima, Natacha Pinheiro Costa Tavares, Chanderlei de Castro de Souza, Rodrigo Otávio Silva Souza, Érica Simplício Souza, João Vicente Braga |
author_sort | Celestino, Jessyca dos Reis |
collection | PubMed |
description | The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening assay of starch hydrolysis in petri dishes (ASHPD) and in the assay in submerged fermentation (ASbF). The strain Aspergillus 6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of Aspergillus 6V4 amylases (385 IU/g) in solid state fermentation (SSF). |
format | Online Article Text |
id | pubmed-3958758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39587582014-04-10 Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate Celestino, Jessyca dos Reis Duarte, Ana Caroline Silva, Cláudia Maria de Melo Sena, Hellen Holanda Ferreira, Maria do Perpétuo Socorro Borges Carriço Mallmann, Neila Hiraishi Lima, Natacha Pinheiro Costa Tavares, Chanderlei de Castro de Souza, Rodrigo Otávio Silva Souza, Érica Simplício Souza, João Vicente Braga Enzyme Res Research Article The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening assay of starch hydrolysis in petri dishes (ASHPD) and in the assay in submerged fermentation (ASbF). The strain Aspergillus 6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of Aspergillus 6V4 amylases (385 IU/g) in solid state fermentation (SSF). Hindawi Publishing Corporation 2014 2014-03-02 /pmc/articles/PMC3958758/ /pubmed/24724017 http://dx.doi.org/10.1155/2014/725651 Text en Copyright © 2014 Jessyca dos Reis Celestino et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Celestino, Jessyca dos Reis Duarte, Ana Caroline Silva, Cláudia Maria de Melo Sena, Hellen Holanda Ferreira, Maria do Perpétuo Socorro Borges Carriço Mallmann, Neila Hiraishi Lima, Natacha Pinheiro Costa Tavares, Chanderlei de Castro de Souza, Rodrigo Otávio Silva Souza, Érica Simplício Souza, João Vicente Braga Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate |
title |
Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by
Using Wheat Bran as a Substrate |
title_full |
Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by
Using Wheat Bran as a Substrate |
title_fullStr |
Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by
Using Wheat Bran as a Substrate |
title_full_unstemmed |
Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by
Using Wheat Bran as a Substrate |
title_short |
Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by
Using Wheat Bran as a Substrate |
title_sort | aspergillus 6v4, a strain isolated from manipueira, produces high amylases levels by
using wheat bran as a substrate |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958758/ https://www.ncbi.nlm.nih.gov/pubmed/24724017 http://dx.doi.org/10.1155/2014/725651 |
work_keys_str_mv | AT celestinojessycadosreis aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT duarteanacaroline aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT silvaclaudiamariademelo aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT senahellenholanda aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT ferreiramariadoperpetuosocorroborgescarrico aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT mallmannneilahiraishi aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT limanatachapinheirocosta aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT tavareschanderleidecastro aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT desouzarodrigootaviosilva aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT souzaericasimplicio aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate AT souzajoaovicentebraga aspergillus6v4astrainisolatedfrommanipueiraproduceshighamylaseslevelsbyusingwheatbranasasubstrate |