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In Silico Identification and Characterization of N-Terminal Acetyltransferase Genes of Poplar (Populus trichocarpa)

N-terminal acetyltransferase (Nats) complex is responsible for protein N-terminal acetylation (Nα-acetylation), which is one of the most common covalent modifications of eukaryotic proteins. Although genome-wide investigation and characterization of Nat catalytic subunits (CS) and auxiliary subunits...

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Autores principales: Zhu, Hang-Yong, Li, Chun-Ming, Wang, Li-Feng, Bai, Hui, Li, Yan-Ping, Yu, Wen-Xi, Xia, De-An, Liu, Chang-Cai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958825/
https://www.ncbi.nlm.nih.gov/pubmed/24473137
http://dx.doi.org/10.3390/ijms15021852
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author Zhu, Hang-Yong
Li, Chun-Ming
Wang, Li-Feng
Bai, Hui
Li, Yan-Ping
Yu, Wen-Xi
Xia, De-An
Liu, Chang-Cai
author_facet Zhu, Hang-Yong
Li, Chun-Ming
Wang, Li-Feng
Bai, Hui
Li, Yan-Ping
Yu, Wen-Xi
Xia, De-An
Liu, Chang-Cai
author_sort Zhu, Hang-Yong
collection PubMed
description N-terminal acetyltransferase (Nats) complex is responsible for protein N-terminal acetylation (Nα-acetylation), which is one of the most common covalent modifications of eukaryotic proteins. Although genome-wide investigation and characterization of Nat catalytic subunits (CS) and auxiliary subunits (AS) have been conducted in yeast and humans they remain unexplored in plants. Here we report on the identification of eleven genes encoding eleven putative Nat CS polypeptides, and five genes encoding five putative Nat AS polypeptides in Populus. We document that the expansion of Nat CS genes occurs as duplicated blocks distributed across 10 of the 19 poplar chromosomes, likely only as a result of segmental duplication events. Based on phylogenetic analysis, poplar Nat CS were assigned to six subgroups, which corresponded well to the Nat CS types (CS of Nat A–F), being consistent with previous reports in humans and yeast. In silico analysis of microarray data showed that in the process of normal development of the poplar, their Nat CS and AS genes are commonly expressed at one relatively low level but share distinct tissue-specific expression patterns. This exhaustive survey of Nat genes in poplar provides important information to assist future studies on their functional role in poplar.
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spelling pubmed-39588252014-03-20 In Silico Identification and Characterization of N-Terminal Acetyltransferase Genes of Poplar (Populus trichocarpa) Zhu, Hang-Yong Li, Chun-Ming Wang, Li-Feng Bai, Hui Li, Yan-Ping Yu, Wen-Xi Xia, De-An Liu, Chang-Cai Int J Mol Sci Article N-terminal acetyltransferase (Nats) complex is responsible for protein N-terminal acetylation (Nα-acetylation), which is one of the most common covalent modifications of eukaryotic proteins. Although genome-wide investigation and characterization of Nat catalytic subunits (CS) and auxiliary subunits (AS) have been conducted in yeast and humans they remain unexplored in plants. Here we report on the identification of eleven genes encoding eleven putative Nat CS polypeptides, and five genes encoding five putative Nat AS polypeptides in Populus. We document that the expansion of Nat CS genes occurs as duplicated blocks distributed across 10 of the 19 poplar chromosomes, likely only as a result of segmental duplication events. Based on phylogenetic analysis, poplar Nat CS were assigned to six subgroups, which corresponded well to the Nat CS types (CS of Nat A–F), being consistent with previous reports in humans and yeast. In silico analysis of microarray data showed that in the process of normal development of the poplar, their Nat CS and AS genes are commonly expressed at one relatively low level but share distinct tissue-specific expression patterns. This exhaustive survey of Nat genes in poplar provides important information to assist future studies on their functional role in poplar. Molecular Diversity Preservation International (MDPI) 2014-01-27 /pmc/articles/PMC3958825/ /pubmed/24473137 http://dx.doi.org/10.3390/ijms15021852 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhu, Hang-Yong
Li, Chun-Ming
Wang, Li-Feng
Bai, Hui
Li, Yan-Ping
Yu, Wen-Xi
Xia, De-An
Liu, Chang-Cai
In Silico Identification and Characterization of N-Terminal Acetyltransferase Genes of Poplar (Populus trichocarpa)
title In Silico Identification and Characterization of N-Terminal Acetyltransferase Genes of Poplar (Populus trichocarpa)
title_full In Silico Identification and Characterization of N-Terminal Acetyltransferase Genes of Poplar (Populus trichocarpa)
title_fullStr In Silico Identification and Characterization of N-Terminal Acetyltransferase Genes of Poplar (Populus trichocarpa)
title_full_unstemmed In Silico Identification and Characterization of N-Terminal Acetyltransferase Genes of Poplar (Populus trichocarpa)
title_short In Silico Identification and Characterization of N-Terminal Acetyltransferase Genes of Poplar (Populus trichocarpa)
title_sort in silico identification and characterization of n-terminal acetyltransferase genes of poplar (populus trichocarpa)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958825/
https://www.ncbi.nlm.nih.gov/pubmed/24473137
http://dx.doi.org/10.3390/ijms15021852
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