Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector

The current study isolated and characterized the Lip3F9 polypeptide sequence of Deschampsia antarctica Desv. (GeneBank Accession Number JX846628), which was found to be comprised of 291 base pairs and was, moreover, expressed in Pichia pastoris X-33 cells. The enzyme was secreted after 24 h of P. pa...

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Autores principales: Rabert, Claudia, Gutiérrez-Moraga, Ana, Navarrete-Gallegos, Alejandro, Navarrete-Campos, Darío, Bravo, León A., Gidekel, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Short Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958855/
https://www.ncbi.nlm.nih.gov/pubmed/24514564
http://dx.doi.org/10.3390/ijms15022359
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author Rabert, Claudia
Gutiérrez-Moraga, Ana
Navarrete-Gallegos, Alejandro
Navarrete-Campos, Darío
Bravo, León A.
Gidekel, Manuel
author_facet Rabert, Claudia
Gutiérrez-Moraga, Ana
Navarrete-Gallegos, Alejandro
Navarrete-Campos, Darío
Bravo, León A.
Gidekel, Manuel
author_sort Rabert, Claudia
collection PubMed
description The current study isolated and characterized the Lip3F9 polypeptide sequence of Deschampsia antarctica Desv. (GeneBank Accession Number JX846628), which was found to be comprised of 291 base pairs and was, moreover, expressed in Pichia pastoris X-33 cells. The enzyme was secreted after 24 h of P. pastoris culture incubation and through induction with methanol. The expressed protein showed maximum lipase activity (35 U/L) with an optimal temperature of 37 °C. The lipase-expressed enzyme lost 50% of its specific activity at 25 °C, a behavior characteristic of a psychrotolerant enzyme. Recombinant enzyme activity was measured in the presence of ionic and non-ionic detergents, and a decrease in enzyme activity was detected for all concentrations of ionic and non-ionic detergents assessed.
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spelling pubmed-39588552014-03-20 Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector Rabert, Claudia Gutiérrez-Moraga, Ana Navarrete-Gallegos, Alejandro Navarrete-Campos, Darío Bravo, León A. Gidekel, Manuel Int J Mol Sci Short Note The current study isolated and characterized the Lip3F9 polypeptide sequence of Deschampsia antarctica Desv. (GeneBank Accession Number JX846628), which was found to be comprised of 291 base pairs and was, moreover, expressed in Pichia pastoris X-33 cells. The enzyme was secreted after 24 h of P. pastoris culture incubation and through induction with methanol. The expressed protein showed maximum lipase activity (35 U/L) with an optimal temperature of 37 °C. The lipase-expressed enzyme lost 50% of its specific activity at 25 °C, a behavior characteristic of a psychrotolerant enzyme. Recombinant enzyme activity was measured in the presence of ionic and non-ionic detergents, and a decrease in enzyme activity was detected for all concentrations of ionic and non-ionic detergents assessed. Molecular Diversity Preservation International (MDPI) 2014-02-07 /pmc/articles/PMC3958855/ /pubmed/24514564 http://dx.doi.org/10.3390/ijms15022359 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Short Note
Rabert, Claudia
Gutiérrez-Moraga, Ana
Navarrete-Gallegos, Alejandro
Navarrete-Campos, Darío
Bravo, León A.
Gidekel, Manuel
Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector
title Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector
title_full Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector
title_fullStr Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector
title_full_unstemmed Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector
title_short Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector
title_sort expression of a deschampsia antarctica desv. polypeptide with lipase activity in a pichia pastoris vector
topic Short Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958855/
https://www.ncbi.nlm.nih.gov/pubmed/24514564
http://dx.doi.org/10.3390/ijms15022359
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