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Interplay between Endothelin and Erythropoietin in Astroglia: The Role in Protection against Hypoxia
We show that, under in vitro conditions, the vulnerability of astroglia to hypoxia is reflected by alterations in endothelin (ET)-1 release and capacity of erythropoietin (EPO) to regulate ET-1 levels. Exposure of cells to 24 h hypoxia did not induce changes in ET-1 release, while 48–72 h hypoxia re...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958886/ https://www.ncbi.nlm.nih.gov/pubmed/24557580 http://dx.doi.org/10.3390/ijms15022858 |
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author | Schäfer, Richard Mueller, Lars Buecheler, Reinhild Proksch, Barbara Schwab, Matthias Gleiter, Christoph H. Danielyan, Lusine |
author_facet | Schäfer, Richard Mueller, Lars Buecheler, Reinhild Proksch, Barbara Schwab, Matthias Gleiter, Christoph H. Danielyan, Lusine |
author_sort | Schäfer, Richard |
collection | PubMed |
description | We show that, under in vitro conditions, the vulnerability of astroglia to hypoxia is reflected by alterations in endothelin (ET)-1 release and capacity of erythropoietin (EPO) to regulate ET-1 levels. Exposure of cells to 24 h hypoxia did not induce changes in ET-1 release, while 48–72 h hypoxia resulted in increase of ET-1 release from astrocytes that could be abolished by EPO. The endothelin receptor type A (ETA) antagonist BQ123 increased extracellular levels of ET-1 in human fetal astroglial cell line (SV-FHAS). The survival and proliferation of rat primary astrocytes, neural precursors, and neurons upon hypoxic conditions were increased upon administration of BQ123. Hypoxic injury and aging affected the interaction between the EPO and ET systems. Under hypoxia EPO decreased ET-1 release from astrocytes, while ETA receptor blockade enhanced the expression of EPO mRNA and EPO receptor in culture-aged rat astroglia. The blockade of ETA receptor can increase the availability of ET-1 to the ETB receptor and can potentiate the neuroprotective effects of EPO. Thus, the new therapeutic use of combined administration of EPO and ETA receptor antagonists during hypoxia-associated neurodegenerative disorders of the central nervous system (CNS) can be suggested. |
format | Online Article Text |
id | pubmed-3958886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-39588862014-03-20 Interplay between Endothelin and Erythropoietin in Astroglia: The Role in Protection against Hypoxia Schäfer, Richard Mueller, Lars Buecheler, Reinhild Proksch, Barbara Schwab, Matthias Gleiter, Christoph H. Danielyan, Lusine Int J Mol Sci Article We show that, under in vitro conditions, the vulnerability of astroglia to hypoxia is reflected by alterations in endothelin (ET)-1 release and capacity of erythropoietin (EPO) to regulate ET-1 levels. Exposure of cells to 24 h hypoxia did not induce changes in ET-1 release, while 48–72 h hypoxia resulted in increase of ET-1 release from astrocytes that could be abolished by EPO. The endothelin receptor type A (ETA) antagonist BQ123 increased extracellular levels of ET-1 in human fetal astroglial cell line (SV-FHAS). The survival and proliferation of rat primary astrocytes, neural precursors, and neurons upon hypoxic conditions were increased upon administration of BQ123. Hypoxic injury and aging affected the interaction between the EPO and ET systems. Under hypoxia EPO decreased ET-1 release from astrocytes, while ETA receptor blockade enhanced the expression of EPO mRNA and EPO receptor in culture-aged rat astroglia. The blockade of ETA receptor can increase the availability of ET-1 to the ETB receptor and can potentiate the neuroprotective effects of EPO. Thus, the new therapeutic use of combined administration of EPO and ETA receptor antagonists during hypoxia-associated neurodegenerative disorders of the central nervous system (CNS) can be suggested. Molecular Diversity Preservation International (MDPI) 2014-02-19 /pmc/articles/PMC3958886/ /pubmed/24557580 http://dx.doi.org/10.3390/ijms15022858 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Schäfer, Richard Mueller, Lars Buecheler, Reinhild Proksch, Barbara Schwab, Matthias Gleiter, Christoph H. Danielyan, Lusine Interplay between Endothelin and Erythropoietin in Astroglia: The Role in Protection against Hypoxia |
title | Interplay between Endothelin and Erythropoietin in Astroglia: The Role in Protection against Hypoxia |
title_full | Interplay between Endothelin and Erythropoietin in Astroglia: The Role in Protection against Hypoxia |
title_fullStr | Interplay between Endothelin and Erythropoietin in Astroglia: The Role in Protection against Hypoxia |
title_full_unstemmed | Interplay between Endothelin and Erythropoietin in Astroglia: The Role in Protection against Hypoxia |
title_short | Interplay between Endothelin and Erythropoietin in Astroglia: The Role in Protection against Hypoxia |
title_sort | interplay between endothelin and erythropoietin in astroglia: the role in protection against hypoxia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958886/ https://www.ncbi.nlm.nih.gov/pubmed/24557580 http://dx.doi.org/10.3390/ijms15022858 |
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