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Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection
Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVC...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3959000/ https://www.ncbi.nlm.nih.gov/pubmed/24672155 http://dx.doi.org/10.4103/0971-6203.121198 |
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author | Shahbazi-Gahrouei, Daryoush Abdolahi, Mohammad |
author_facet | Shahbazi-Gahrouei, Daryoush Abdolahi, Mohammad |
author_sort | Shahbazi-Gahrouei, Daryoush |
collection | PubMed |
description | Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell. Magnetic resonance imaging parameters of the prepared nanoconjugate was investigated in vitro: characterization, cell toxicity, flow cytometry, Prussian blue staining, and cellular uptake as well as biodistribution and magnetic resonance signal intensities under in vivo conditions were also investigated. Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer. In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool. Further subsequent in vivo studies and clinical trials are warranted. |
format | Online Article Text |
id | pubmed-3959000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-39590002014-03-26 Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection Shahbazi-Gahrouei, Daryoush Abdolahi, Mohammad J Med Phys Original Article Superparamagnetic iron oxide nanoparticles (SPIONs), have played an important role in the promotion of image contrast in magnetic resonance imaging modality. The objective of present study is describing SPIONs conjugated with C595 monoclonal antibody (mAb) against MUC1-expressing ovarian cancer (OVCAR3) cell. Magnetic resonance imaging parameters of the prepared nanoconjugate was investigated in vitro: characterization, cell toxicity, flow cytometry, Prussian blue staining, and cellular uptake as well as biodistribution and magnetic resonance signal intensities under in vivo conditions were also investigated. Magnetic resonance imaging and biodistribution results showed good tumor accumulation and detection, no cytotoxicity, and potential selective as anti-ovarian cancer. In conclusion, based on the findings SPIONs-C595 nanosized-probe is potentially, a selective ovarian molecular imaging tool. Further subsequent in vivo studies and clinical trials are warranted. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3959000/ /pubmed/24672155 http://dx.doi.org/10.4103/0971-6203.121198 Text en Copyright: © Journal of Medical Physics http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Shahbazi-Gahrouei, Daryoush Abdolahi, Mohammad Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection |
title | Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection |
title_full | Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection |
title_fullStr | Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection |
title_full_unstemmed | Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection |
title_short | Superparamagnetic iron oxide-C595: Potential MR imaging contrast agents for ovarian cancer detection |
title_sort | superparamagnetic iron oxide-c595: potential mr imaging contrast agents for ovarian cancer detection |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3959000/ https://www.ncbi.nlm.nih.gov/pubmed/24672155 http://dx.doi.org/10.4103/0971-6203.121198 |
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