Simple and sure methodology for massive hepatectomy in the mouse

BACKGROUND: Reliable models for massive hepatectomy in the mouse are required for experimental liver research. METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction >70%. The impact of various factors in the different models was also analyze...

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Detalles Bibliográficos
Autores principales: Hori, Tomohide, Ohashi, Norifumi, Chen, Feng, Baine, Ann-Marie T., Gardner, Lindsay B., Jermanus, Sura, Nguyen, Justin H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hellenic Society of Gastroenterology 2011
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3959334/
https://www.ncbi.nlm.nih.gov/pubmed/24713791
Descripción
Sumario:BACKGROUND: Reliable models for massive hepatectomy in the mouse are required for experimental liver research. METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction >70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves and histopathological findings. RESULTS: According to anatomical results, murine models with 75%, 80% and 90% of liver resection produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques. CONCLUSION: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.

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