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NTRK1 Fusion in Glioblastoma Multiforme
Glioblastoma multiforme (GBM) is the most aggressive form of brain tumor, yet with no targeted therapy with substantial survival benefit. Recent studies on solid tumors showed that fusion genes often play driver roles and are promising targets for pharmaceutical intervention. To survey potential fus...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960150/ https://www.ncbi.nlm.nih.gov/pubmed/24647444 http://dx.doi.org/10.1371/journal.pone.0091940 |
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author | Kim, Jinkuk Lee, Yeri Cho, Hee-Jin Lee, Young-Eun An, Jaeyeol Cho, Gye-Hyun Ko, Young-Hyeh Joo, Kyeung Min Nam, Do-Hyun |
author_facet | Kim, Jinkuk Lee, Yeri Cho, Hee-Jin Lee, Young-Eun An, Jaeyeol Cho, Gye-Hyun Ko, Young-Hyeh Joo, Kyeung Min Nam, Do-Hyun |
author_sort | Kim, Jinkuk |
collection | PubMed |
description | Glioblastoma multiforme (GBM) is the most aggressive form of brain tumor, yet with no targeted therapy with substantial survival benefit. Recent studies on solid tumors showed that fusion genes often play driver roles and are promising targets for pharmaceutical intervention. To survey potential fusion genes in GBMs, we analysed RNA-Seq data from 162 GBM patients available through The Cancer Genome Atlas (TCGA), and found that 3′ exons of neurotrophic tyrosine kinase receptor type 1 (NTRK1, encoding TrkA) are fused to 5′ exons of the genes that are highly expressed in neuronal tissues, neurofascin (NFASC) and brevican (BCAN). The fusions preserved both the transmembrane and kinase domains of NTRK1 in frame. NTRK1 is a mediator of the pro-survival signaling of nerve growth factor (NGF) and is a known oncogene, found commonly altered in human cancer. While GBMs largely lacked NTRK1 expression, the fusion-positive GBMs expressed fusion transcripts in high abundance, and showed elevated NTRK1-pathway activity. Lentiviral transduction of the NFASC-NTRK1 fusion gene in NIH 3T3 cells increased proliferation in vitro, colony formation in soft agar, and tumor formation in mice, suggesting the possibility that the fusion contributed to the initiation or maintenance of the fusion-positive GBMs, and therefore may be a rational drug target. |
format | Online Article Text |
id | pubmed-3960150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39601502014-03-27 NTRK1 Fusion in Glioblastoma Multiforme Kim, Jinkuk Lee, Yeri Cho, Hee-Jin Lee, Young-Eun An, Jaeyeol Cho, Gye-Hyun Ko, Young-Hyeh Joo, Kyeung Min Nam, Do-Hyun PLoS One Research Article Glioblastoma multiforme (GBM) is the most aggressive form of brain tumor, yet with no targeted therapy with substantial survival benefit. Recent studies on solid tumors showed that fusion genes often play driver roles and are promising targets for pharmaceutical intervention. To survey potential fusion genes in GBMs, we analysed RNA-Seq data from 162 GBM patients available through The Cancer Genome Atlas (TCGA), and found that 3′ exons of neurotrophic tyrosine kinase receptor type 1 (NTRK1, encoding TrkA) are fused to 5′ exons of the genes that are highly expressed in neuronal tissues, neurofascin (NFASC) and brevican (BCAN). The fusions preserved both the transmembrane and kinase domains of NTRK1 in frame. NTRK1 is a mediator of the pro-survival signaling of nerve growth factor (NGF) and is a known oncogene, found commonly altered in human cancer. While GBMs largely lacked NTRK1 expression, the fusion-positive GBMs expressed fusion transcripts in high abundance, and showed elevated NTRK1-pathway activity. Lentiviral transduction of the NFASC-NTRK1 fusion gene in NIH 3T3 cells increased proliferation in vitro, colony formation in soft agar, and tumor formation in mice, suggesting the possibility that the fusion contributed to the initiation or maintenance of the fusion-positive GBMs, and therefore may be a rational drug target. Public Library of Science 2014-03-19 /pmc/articles/PMC3960150/ /pubmed/24647444 http://dx.doi.org/10.1371/journal.pone.0091940 Text en © 2014 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kim, Jinkuk Lee, Yeri Cho, Hee-Jin Lee, Young-Eun An, Jaeyeol Cho, Gye-Hyun Ko, Young-Hyeh Joo, Kyeung Min Nam, Do-Hyun NTRK1 Fusion in Glioblastoma Multiforme |
title |
NTRK1 Fusion in Glioblastoma Multiforme |
title_full |
NTRK1 Fusion in Glioblastoma Multiforme |
title_fullStr |
NTRK1 Fusion in Glioblastoma Multiforme |
title_full_unstemmed |
NTRK1 Fusion in Glioblastoma Multiforme |
title_short |
NTRK1 Fusion in Glioblastoma Multiforme |
title_sort | ntrk1 fusion in glioblastoma multiforme |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960150/ https://www.ncbi.nlm.nih.gov/pubmed/24647444 http://dx.doi.org/10.1371/journal.pone.0091940 |
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