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FOXP3 over-expression inhibits melanoma tumorigenesis via effects on proliferation and apoptosis.

The Forkhead box P3 (FOXP3) transcription factor is the key driver of regulatory T cell (Treg cells) differentiation and immunosuppressive function. In addition, FOXP3 has been reported to be expressed in many tumors, including melanoma. However, its role in tumorigenesis is conficting, with both tu...

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Autores principales: Tan, BeeShin, Anaka, Matthew, Deb, Siddhartha, Freyer, Claudia, Ebert, Lisa M., Chueh, Anderly C., Al-Obaidi, Sheren, Behren, Andreas, Jayachandran, Aparna, Cebon, Jonathan, Chen, Weisan, Mariadason, John M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960207/
https://www.ncbi.nlm.nih.gov/pubmed/24406338
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author Tan, BeeShin
Anaka, Matthew
Deb, Siddhartha
Freyer, Claudia
Ebert, Lisa M.
Chueh, Anderly C.
Al-Obaidi, Sheren
Behren, Andreas
Jayachandran, Aparna
Cebon, Jonathan
Chen, Weisan
Mariadason, John M.
author_facet Tan, BeeShin
Anaka, Matthew
Deb, Siddhartha
Freyer, Claudia
Ebert, Lisa M.
Chueh, Anderly C.
Al-Obaidi, Sheren
Behren, Andreas
Jayachandran, Aparna
Cebon, Jonathan
Chen, Weisan
Mariadason, John M.
author_sort Tan, BeeShin
collection PubMed
description The Forkhead box P3 (FOXP3) transcription factor is the key driver of regulatory T cell (Treg cells) differentiation and immunosuppressive function. In addition, FOXP3 has been reported to be expressed in many tumors, including melanoma. However, its role in tumorigenesis is conficting, with both tumor suppressive and tumor promoting functions described. The aim of the current study was to characterize the expression and function of FOXP3 in melanoma. FOXP3 expression was detected by immunohistochemistry (IHC) in 12% (18/146) of stage III and IV melanomas. However expression was confined to fewer than 1% of cells in these tumors. Stable over-expression of FOXP3 in the SK-MEL-28 melanoma cell line reduced cell proliferation and clonogenicity in vitro, and reduced xenograft growth in vivo. FOXP3 over-expression also increased pigmentation and the rate of apoptosis of SK-MEL-28 cells. Based on its infrequent expression in human melanoma, and its growth inhibitory and pro-apoptotic effect in over-expressing melanoma cells, we conclude that FOXP3 is not likely to be a key tumor suppressor or promoter in melanoma.
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spelling pubmed-39602072014-04-04 FOXP3 over-expression inhibits melanoma tumorigenesis via effects on proliferation and apoptosis. Tan, BeeShin Anaka, Matthew Deb, Siddhartha Freyer, Claudia Ebert, Lisa M. Chueh, Anderly C. Al-Obaidi, Sheren Behren, Andreas Jayachandran, Aparna Cebon, Jonathan Chen, Weisan Mariadason, John M. Oncotarget Research Paper The Forkhead box P3 (FOXP3) transcription factor is the key driver of regulatory T cell (Treg cells) differentiation and immunosuppressive function. In addition, FOXP3 has been reported to be expressed in many tumors, including melanoma. However, its role in tumorigenesis is conficting, with both tumor suppressive and tumor promoting functions described. The aim of the current study was to characterize the expression and function of FOXP3 in melanoma. FOXP3 expression was detected by immunohistochemistry (IHC) in 12% (18/146) of stage III and IV melanomas. However expression was confined to fewer than 1% of cells in these tumors. Stable over-expression of FOXP3 in the SK-MEL-28 melanoma cell line reduced cell proliferation and clonogenicity in vitro, and reduced xenograft growth in vivo. FOXP3 over-expression also increased pigmentation and the rate of apoptosis of SK-MEL-28 cells. Based on its infrequent expression in human melanoma, and its growth inhibitory and pro-apoptotic effect in over-expressing melanoma cells, we conclude that FOXP3 is not likely to be a key tumor suppressor or promoter in melanoma. Impact Journals LLC 2013-12-20 /pmc/articles/PMC3960207/ /pubmed/24406338 Text en Copyright: © 2014 Tan et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Tan, BeeShin
Anaka, Matthew
Deb, Siddhartha
Freyer, Claudia
Ebert, Lisa M.
Chueh, Anderly C.
Al-Obaidi, Sheren
Behren, Andreas
Jayachandran, Aparna
Cebon, Jonathan
Chen, Weisan
Mariadason, John M.
FOXP3 over-expression inhibits melanoma tumorigenesis via effects on proliferation and apoptosis.
title FOXP3 over-expression inhibits melanoma tumorigenesis via effects on proliferation and apoptosis.
title_full FOXP3 over-expression inhibits melanoma tumorigenesis via effects on proliferation and apoptosis.
title_fullStr FOXP3 over-expression inhibits melanoma tumorigenesis via effects on proliferation and apoptosis.
title_full_unstemmed FOXP3 over-expression inhibits melanoma tumorigenesis via effects on proliferation and apoptosis.
title_short FOXP3 over-expression inhibits melanoma tumorigenesis via effects on proliferation and apoptosis.
title_sort foxp3 over-expression inhibits melanoma tumorigenesis via effects on proliferation and apoptosis.
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960207/
https://www.ncbi.nlm.nih.gov/pubmed/24406338
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