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The BDNF effects on dendritic spines of mature hippocampal neurons depend on neuronal activity
The fine tuning of neural networks during development and learning relies upon both functional and structural plastic processes. Changes in the number as well as in the size and shape of dendritic spines are associated to long-term activity-dependent synaptic plasticity. However, the molecular mecha...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960490/ https://www.ncbi.nlm.nih.gov/pubmed/24688467 http://dx.doi.org/10.3389/fnsyn.2014.00005 |
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author | Kellner, Yves Gödecke, Nina Dierkes, Tobias Thieme, Nils Zagrebelsky, Marta Korte, Martin |
author_facet | Kellner, Yves Gödecke, Nina Dierkes, Tobias Thieme, Nils Zagrebelsky, Marta Korte, Martin |
author_sort | Kellner, Yves |
collection | PubMed |
description | The fine tuning of neural networks during development and learning relies upon both functional and structural plastic processes. Changes in the number as well as in the size and shape of dendritic spines are associated to long-term activity-dependent synaptic plasticity. However, the molecular mechanisms translating functional into structural changes are still largely unknown. In this context, neurotrophins, like Brain-Derived Neurotrophic Factor (BDNF), are among promising candidates. Specifically BDNF-TrkB receptor signaling is crucial for activity-dependent strengthening of synapses in different brain regions. BDNF application has been shown to positively modulate dendritic and spine architecture in cortical and hippocampal neurons as well as structural plasticity in vitro. However, a global BDNF deprivation throughout the central nervous system (CNS) resulted in very mild structural alterations of dendritic spines, questioning the relevance of the endogenous BDNF signaling in modulating the development and the mature structure of neurons in vivo. Here we show that a loss-of-function approach, blocking BDNF results in a significant reduction in dendritic spine density, associated with an increase in spine length and a decrease in head width. These changes are associated with a decrease in F-actin levels within spine heads. On the other hand, a gain-of-function approach, applying exogenous BDNF, could not reproduce the increase in spine density or the changes in spine morphology previously described. Taken together, we show here that the effects exerted by BDNF on the dendritic architecture of hippocampal neurons are dependent on the neuron's maturation stage. Indeed, in mature hippocampal neurons in vitro as shown in vivo BDNF is specifically required for the activity-dependent maintenance of the mature spine phenotype. |
format | Online Article Text |
id | pubmed-3960490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-39604902014-03-31 The BDNF effects on dendritic spines of mature hippocampal neurons depend on neuronal activity Kellner, Yves Gödecke, Nina Dierkes, Tobias Thieme, Nils Zagrebelsky, Marta Korte, Martin Front Synaptic Neurosci Neuroscience The fine tuning of neural networks during development and learning relies upon both functional and structural plastic processes. Changes in the number as well as in the size and shape of dendritic spines are associated to long-term activity-dependent synaptic plasticity. However, the molecular mechanisms translating functional into structural changes are still largely unknown. In this context, neurotrophins, like Brain-Derived Neurotrophic Factor (BDNF), are among promising candidates. Specifically BDNF-TrkB receptor signaling is crucial for activity-dependent strengthening of synapses in different brain regions. BDNF application has been shown to positively modulate dendritic and spine architecture in cortical and hippocampal neurons as well as structural plasticity in vitro. However, a global BDNF deprivation throughout the central nervous system (CNS) resulted in very mild structural alterations of dendritic spines, questioning the relevance of the endogenous BDNF signaling in modulating the development and the mature structure of neurons in vivo. Here we show that a loss-of-function approach, blocking BDNF results in a significant reduction in dendritic spine density, associated with an increase in spine length and a decrease in head width. These changes are associated with a decrease in F-actin levels within spine heads. On the other hand, a gain-of-function approach, applying exogenous BDNF, could not reproduce the increase in spine density or the changes in spine morphology previously described. Taken together, we show here that the effects exerted by BDNF on the dendritic architecture of hippocampal neurons are dependent on the neuron's maturation stage. Indeed, in mature hippocampal neurons in vitro as shown in vivo BDNF is specifically required for the activity-dependent maintenance of the mature spine phenotype. Frontiers Media S.A. 2014-03-20 /pmc/articles/PMC3960490/ /pubmed/24688467 http://dx.doi.org/10.3389/fnsyn.2014.00005 Text en Copyright © 2014 Kellner, Gödecke, Dierkes, Thieme, Zagrebelsky and Korte. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Kellner, Yves Gödecke, Nina Dierkes, Tobias Thieme, Nils Zagrebelsky, Marta Korte, Martin The BDNF effects on dendritic spines of mature hippocampal neurons depend on neuronal activity |
title | The BDNF effects on dendritic spines of mature hippocampal neurons depend on neuronal activity |
title_full | The BDNF effects on dendritic spines of mature hippocampal neurons depend on neuronal activity |
title_fullStr | The BDNF effects on dendritic spines of mature hippocampal neurons depend on neuronal activity |
title_full_unstemmed | The BDNF effects on dendritic spines of mature hippocampal neurons depend on neuronal activity |
title_short | The BDNF effects on dendritic spines of mature hippocampal neurons depend on neuronal activity |
title_sort | bdnf effects on dendritic spines of mature hippocampal neurons depend on neuronal activity |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960490/ https://www.ncbi.nlm.nih.gov/pubmed/24688467 http://dx.doi.org/10.3389/fnsyn.2014.00005 |
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