Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice

Inflamm-aging indicates the chronic inflammatory state resulting from increased secretion of proinflammatory cytokines and mediators such as IL-6 in the elderly. Our principle objective was to identify cell types that were affected with aging concerning IL-6 secretion in the murine model. We compare...

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Detalles Bibliográficos
Autores principales: Park, Jihyun, Miyakawa, Takuya, Shiokawa, Aya, Nakajima-Adachi, Haruyo, Tanokura, Masaru, Hachimura, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960767/
https://www.ncbi.nlm.nih.gov/pubmed/24729663
http://dx.doi.org/10.1155/2014/826987
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author Park, Jihyun
Miyakawa, Takuya
Shiokawa, Aya
Nakajima-Adachi, Haruyo
Tanokura, Masaru
Hachimura, Satoshi
author_facet Park, Jihyun
Miyakawa, Takuya
Shiokawa, Aya
Nakajima-Adachi, Haruyo
Tanokura, Masaru
Hachimura, Satoshi
author_sort Park, Jihyun
collection PubMed
description Inflamm-aging indicates the chronic inflammatory state resulting from increased secretion of proinflammatory cytokines and mediators such as IL-6 in the elderly. Our principle objective was to identify cell types that were affected with aging concerning IL-6 secretion in the murine model. We compared IL-6 production in spleen cells from both young and aged mice and isolated several types of cells from spleen and investigated IL-6 mRNA expression and protein production. IL-6 protein productions in cultured stromal cells from aged mice spleen were significantly high compared to young mice upon LPS stimulation. IL-6 mRNA expression level of freshly isolated stromal cells from aged mice was high compared to young mice. Furthermore, stromal cells of aged mice highly expressed IL-6 mRNA after LPS injection in vivo. These results suggest that stromal cells play a role in producing IL-6 in aged mice and imply that they contribute to the chronic inflammatory condition in the elderly.
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spelling pubmed-39607672014-04-13 Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice Park, Jihyun Miyakawa, Takuya Shiokawa, Aya Nakajima-Adachi, Haruyo Tanokura, Masaru Hachimura, Satoshi Mediators Inflamm Research Article Inflamm-aging indicates the chronic inflammatory state resulting from increased secretion of proinflammatory cytokines and mediators such as IL-6 in the elderly. Our principle objective was to identify cell types that were affected with aging concerning IL-6 secretion in the murine model. We compared IL-6 production in spleen cells from both young and aged mice and isolated several types of cells from spleen and investigated IL-6 mRNA expression and protein production. IL-6 protein productions in cultured stromal cells from aged mice spleen were significantly high compared to young mice upon LPS stimulation. IL-6 mRNA expression level of freshly isolated stromal cells from aged mice was high compared to young mice. Furthermore, stromal cells of aged mice highly expressed IL-6 mRNA after LPS injection in vivo. These results suggest that stromal cells play a role in producing IL-6 in aged mice and imply that they contribute to the chronic inflammatory condition in the elderly. Hindawi Publishing Corporation 2014 2014-03-04 /pmc/articles/PMC3960767/ /pubmed/24729663 http://dx.doi.org/10.1155/2014/826987 Text en Copyright © 2014 Jihyun Park et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Park, Jihyun
Miyakawa, Takuya
Shiokawa, Aya
Nakajima-Adachi, Haruyo
Tanokura, Masaru
Hachimura, Satoshi
Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice
title Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice
title_full Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice
title_fullStr Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice
title_full_unstemmed Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice
title_short Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice
title_sort splenic stromal cells from aged mice produce higher levels of il-6 compared to young mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960767/
https://www.ncbi.nlm.nih.gov/pubmed/24729663
http://dx.doi.org/10.1155/2014/826987
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