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Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Ideal cell-free expression systems can theoretically emulate an in vivo cellular environment in a controlled in vitro platform.(1) This is useful for expressing proteins and genetic circuits in a controlled manner as well as for providing a prototyping environment for synthetic biology.(2,3 ) To ach...

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Detalles Bibliográficos
Autores principales:	Sun, Zachary Z., Hayes, Clarmyra A., Shin, Jonghyeon, Caschera, Filippo, Murray, Richard M., Noireaux, Vincent
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2013
Materias:	Cellular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960857/ https://www.ncbi.nlm.nih.gov/pubmed/24084388 http://dx.doi.org/10.3791/50762

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